ResearchCalibration of intracellular Ca transients of isolated adult heart cells labelled with fura-2 by acetoxymethyl ester loading
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Cited by (37)
Differential subcellular Ca<sup>2+</sup> signaling in a highly specialized subpopulation of astrocytes
2015, Experimental NeurologySalvia miltiorrhiza (Danshen) inhibits L-type calcium current and attenuates calcium transient and contractility in rat ventricular myocytes
2014, Journal of EthnopharmacologyCitation Excerpt :Qualitative changes in [Ca2+]i levels were inferred from the ratio of the fluorescence intensity at two wavelengths (340/380 nm) (Howarth et al., 2002; Salem et al., 2010; Ren et al., 2012). Ca2+ transients were calibrated as described previously (Haworth and Redon, 1998). Collagenase type II was obtained from Gibco.
Synthesis and properties of Asante Calcium Red-A novel family of long excitation wavelength calcium indicators
2013, Cell CalciumCitation Excerpt :Transition metal ions, Co2+ and, somewhat less effectively, Mn2+, displace calcium bound to ACR-1 and reduce its fluorescence (Supplemental Figure 6). This feature might allow using previously developed quenching-based protocols to calibrate [56–58] and characterize ACR-1 and ACR-1-LA properties in situ [59–62]. To assess the impact of H+ binding on ACR-1 fluorescence, we recorded indicator emission spectra in Ca2+-free (10 mM EGTA) calibration buffers with pH ranging from 6.0 to 7.8 (Fig. 5).
The effect of ligustrazine on L-type calcium current, calcium transient and contractility in rabbit ventricular myocytes
2012, Journal of EthnopharmacologyCitation Excerpt :Qualitative changes in intracellular Ca2+ levels were inferred from the ratio of the fluorescence intensity at two wavelengths (340/380) and were presented as fura-2 fluorescence intensity (FFI). Calcium transients were calibrated as described previously (Haworth and Redon, 1998). Whole-cell recordings were analyzed using FitMaster(v2x32, HEKA).
Down-regulation of Homer1b/c attenuates glutamate-mediated excitotoxicity through endoplasmic reticulum and mitochondria pathways in rat cortical neurons
2012, Free Radical Biology and MedicineCitation Excerpt :Images were collected and analyzed with the MetaFluor image-processing software. The [Ca2 +]cyt and [Ca2 +]ER values were then calculated and the Ca2 +-insensitive fluorescence was subtracted from each wavelength before calculations [21]. Double-stranded small interfering RNA (siRNA) transfection was used to knock down Homer1b/c expression.
Simultaneous measurements of mitochondrial NADH and Ca<sup>2+</sup> during increased work in intact rat heart trabeculae
2002, Biophysical JournalCitation Excerpt :As shown here, the quenching of mitochondrial Rhod-2 occurred so fast that it was not possible to selectively exclude cytosolic Rhod-2 from mitochondrial Rhod-2 at any time after Mn2+ application. This appears to be in contrast to the studies using Indo-1 in hearts (Schreur et al., 1996; Griffiths et al., 1997a), where cytosolic Indo-1 was significantly quenched before mitochondrial Indo-1, but in accordance with studies using Fura-2 (Haworth and Redon, 1998). To explain the different results in the different studies, it is possible that there were differences in mitochondrial Mn2+ uptake rates (e.g., due to differing membrane potential) or that there were differences in the kinetics of Mn2+ binding to mitochondrial versus cytosolic dye.