Elsevier

Neuroscience Letters

Volume 287, Issue 1, 16 June 2000, Pages 33-36
Neuroscience Letters

Prostaglandin E2-mediated sensitization of rat sensory neurons is not altered by nerve growth factor

https://doi.org/10.1016/S0304-3940(00)01158-7Get rights and content

Abstract

To ascertain whether chronic exposure to nerve growth factor (NGF) alters the responsiveness of sensory neurons to prostaglandin E2 (PGE2), sensory neurons taken from adult rats were grown in culture in the presence or absence of NGF for 7 days. Neurons then were exposed to PGE2 and release of immunoreactive calcitonin gene-related peptide (iCGRP) and production of immunoreactive cAMP (icAMP) were examined. Growing neurons in the presence of 250 ng/ml NGF increased the content and the release of iCGRP from sensory neurons. Independent of NGF treatment, exposure to 100 nM PGE2 augmented capsaicin- or potassium-stimulated release of iCGRP by 1.5-fold compared with cells not exposed to PGE2. In a similar manner, NGF treatment did not alter the ability of PGE2 to increase the content of icAMP. These data suggest that prostaglandin-induced sensitization of sensory neurons is not influenced by NGF.

Section snippets

Acknowledgements

This work was supported by PHS 1-RO1 NS 34159 to M.R.V. M.D.S was supported by a fellowship from American Heart Association, Indiana Affiliate.

References (20)

There are more references available in the full text version of this article.

Cited by (30)

  • Neural communication in the trigeminal ganglion contributes to ectopic orofacial pain

    2013, Journal of Oral Biosciences
    Citation Excerpt :

    CGRP-positive TG neurons in small-cell groups innervating whisker pad skin significantly increased after lower lip inflammation [56]. NGF injection into skin results in increases in the expression of CGRP in the DRG [57], and it increases mRNA and CGRP levels in DRG cultures [58,59]. These findings suggest that NGF produced in the lower lip locally after CFA injection is involved in the up-regulation of CGRP in TG neurons.

  • Capsaicin-evoked iCGRP release from human dental pulp: A model system for the study of peripheral neuropeptide secretion in normal healthy tissue

    2009, Pain
    Citation Excerpt :

    Receptors for PGE2 are expressed in human dental pulp [19], thus we also examined the effects of PGE2 on iCGRP release. Although we observed a small direct stimulation with PGE2 treatment, we did not detect sensitization of capsaicin-stimulated release by PGE2 as observed from sensory neurons in culture and from spinal cord [39,69,80]. The mu-opioid receptor (MOR) is also present on nerve fibers in the dental pulp [41], therefore we examined the ability of our assay to detect inhibition of capsaicin-stimulated iCGRP release with the MOR agonist, DAMGO.

  • Glial cell line-derived neurotrophic factor family ligands enhance capsaicin-stimulated release of calcitonin gene-related peptide from sensory neurons

    2009, Neuroscience
    Citation Excerpt :

    PGE2 is a well-established sensory neuronal sensitizing agent (Martin et al., 1987; Mense, 1981). It is known to sensitize sensory neurons to many stimuli, including high extracellular potassium (Southall and Vasko, 2000). Accordingly, PGE2 enhanced the potassium-stimulated release of iCGRP by nearly twofold (Fig. 4; 14.3%±0.79%).

View all citing articles on Scopus
View full text