Anisomycin inhibits the late maintenance of long-term depression in rat hippocampal slices in vitro

doi:10.1016/S0304-3940(02)01400-3

Neuroscience Letters

Volume 338, Issue 2, 27 February 2003, Pages 147-150

https://doi.org/10.1016/S0304-3940(02)01400-3 Get rights and content

Abstract

Studies were performed to investigate whether electrically-induced long-term depression (LTD) within rat hippocampal slices in vitro shares any common cellular features with LTD in the intact animal, with particular emphasis being placed on mechanisms required for its late maintenance. Our initial studies have led to the development of stimulation protocols which are able to reliably produce different forms of LTD. Depending on the induction protocol applied, we are able to demonstrate a transient protein synthesis-independent early-LTD with a duration of up to 3–4 h, together with a de novo protein synthesis-dependent late-LTD lasting for at least 8 h. Furthermore, we are able to show input-specific LTD within the CA1 region, with expression shown only by those synapses specifically stimulated by a low-frequency protocol. These studies are important pre-requisites to investigate mechanisms of ‘synaptic tagging’ and ‘late-associativity’ during LTD.

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Acknowledgements

We thank Dr Jason Pycrof for his critical comments on the manuscript. This work was supported by a grant of the VolkswagenStiftung I/77 922 to J.U.F.

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Anisomycin inhibits the late maintenance of long-term depression in rat hippocampal slices in vitro

Abstract

Section snippets

Acknowledgements

Curr. Opin. Neurobiol.

Brain Res.

Trends Neurosci.

Neuropharmacology

Brain Res. Bull.

Prog. Neurobiol.

Neuron

Neuropharmacology