Elsevier

Neuroscience Letters

Volume 206, Issues 2–3, 15 March 1996, Pages 161-164
Neuroscience Letters

Evidence for colocalization of GABA and glycine in afferents to retrogradely labelled rat abducens motoneurones

https://doi.org/10.1016/S0304-3940(96)12465-4Get rights and content

Abstract

The coexistence of γ-aminobutyric acid (GABA) and glycine in axon terminals impinging on rat abducens motoneurones was investigated using a double staining procedure combining retrograde labelling of the motoneurones with HRP and post-embedding immunocytochemical staining of axon terminals. Adjacent ultrathin sections of cell bodies of identified motoneurones were individually treated with GABA or glycine antibodies. The terminals single labelled for GABA represented 11.4% of the terminals analyzed, while 8% of them were glycine immunoreactive and 9% were both GABA and glycine immunoreactive. All the labelled terminals contained pleomorphic vesicles. The mean length of apposition of the double labelled terminals was statistically larger (2.20 ± 0.97 μm) than the GABA (1.65 ± 0.57 μm) or glycine immunoreactive ones (1.37 ± 0.35 μm).

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    We wish to thank Dr N. Chmykhova for critical reading of the manuscript.

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