Differential immunoreactivity for alpha-actinin-2, an N-methyl-d-aspartate-receptor/actin binding protein, in hippocampal interneurons
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Animals
Adult and juvenile Wistar rats (adult 150–250 g, juvenile 10–50 g; Simonsen, Gilroy, CA, USA) were used in this study. All animals were deeply anesthetized with sodium pentobarbital (100 mg/kg) and transcardially perfused with one of the fixatives described below. For the developmental study, pups aged P1, P7, P13, and P21 were used. All other tissue was from control adult rats unless noted. All experiments were performed in accordance with protocols approved by the University of California,
Interneuronal somata in the dentate gyrus and CA1 are highly immunoreactive for α-actinin-2
A previous study described α-actinin-2 staining throughout the hippocampal formation, including high immunoreactivity in CA2, heavy staining of the molecular layer of the dentate gyrus, and distinct immunoreactivity of scattered, presumed interneurons.80 A similar distribution pattern for α-actinin-2 was seen in this study (Fig. 1a). The highest level of α-actinin-2 immunoreactivity was seen in the molecular layer of the dentate gyrus and in CA2 neurons. The neuropil of the hilus and CA3
Differential immunoreactivity for α-actinin-2 in principal cells and interneurons
This study demonstrates differential immunoreactivity for α-actinin-2 in the somata and dendrites of interneurons compared to the mostly dendritic immunoreactivity in principal cells of CA1 and the dentate gyrus. For all double-label experiments, cells were counted bidirectionally (first to assess marker labeling in the α-actinin-2-positive cells and secondly to assess α-actinin-2 labeling in the marker-positive cells) to minimize bias in assessing double-stained neurons. In the cases where
Acknowledgements
This work was supported by the NIH (grant NS35915 to I.S.). A. d. H. R. was supported by the NIH (grant T32 GM08620 to the University of California, Irvine M.D./Ph.D. training program). The authors thank Dr R. T. Robertson and Dr D. O'Dowd for usage of their fluorescent microscopes and Ms. J. Baratta, Ms. V. Santhakumar and Ms. R. Zhu for expert technical assistance.
References (85)
- et al.
Immunohistochemical localization of calcium-binding protein in the cerebellum, hippocampal formation and olfactory bulb of the rat
Brain Res.
(1982) - et al.
Changes in voltage dependence of NMDA currents during development
Neurosci. Lett.
(1988) - et al.
GABAA, NMDA and AMPA receptors: a developmentally regulated ‘ménage à trois’
Trends Neurosci.
(1997) - et al.
Distribution and co-localization of calbindin D28k with VIP and neuropeptide Y but not somatostatin, galanin and substance P in the enteric nervous system of the rat
Peptides
(1988) - et al.
Cellular and subcellular localization of the 2B-subunit of the NMDA receptor in the adult rat telencephalon
Brain Res.
(1999) - et al.
GABA: an excitatory transmitter in early postnatal life
Trends Neurosci.
(1991) - et al.
Distribution of neuropeptide Y-like immunoreactivity in the rat central nervous system—II. Immunohistochemical analysis
Neuroscience
(1986) - et al.
Structure of the alpha-actinin rod: molecular basis for cross-linking of actin filaments
Cell
(1999) - et al.
Innervation of different peptide-containing neurons in the hippocampus by GABAergic septal afferents
Neuroscience
(1990) - et al.
Calretinin is present in non-pyramidal cells of the rat hippocampus—I. A new type of neuron specifically associated with the mossy fibre system
Neuroscience
(1992)
Co-localization of neuropeptide tyrosine and somatostatin immunoreactivity in neurons of individual subfields of the rat hippocampal region
Neurosci. Lett.
AMPA receptors in the rat and primate hippocampus: a possible absence of GluR2/3 subunits in most interneurons
Neuroscience
Neurogenesis of GABAergic neurons in the rat dentate gyrus: a combined autoradiographic and immunocytochemical study
Neurosci. Lett.
Passive propagation of LTD to stratum oriens-alveus inhibitory neurons modulates the temporoammonic input to the hippocampal CA1 region
Neuron
Postsynaptic actin and neuronal plasticity
Curr. Opin. Neurobiol.
Traumatic brain injury in the rat: characterization of a lateral fluid-percussion model
Neuroscience
Bridging the cleft at GABA synapses in the brain
Trends Neurosci.
Reduced sensitivity of the N-methyl-d-aspartate component of synaptic transmission to magnesium in hippocampal slices from immature rats
Devl Brain Res.
Localization and seizure-regulation of integrin beta 1 mRNA in adult rat brain
Molec. Brain Res.
Multiple receptors for neuropeptide Y in the hippocampus: putative roles in seizures and cognition
Brain Res.
Calcium-induced actin depolymerization reduces NMDA channel activity
Neuron
Neuropeptides-immunoreactivity and their mRNA expression in kindling: functional implications for limbic epileptogenesis
Brain Res. Rev.
Calretinin immunoreactivity in the monkey hippocampal formation—I. Light and electron microscopic characteristics and co-localization with other calcium-binding proteins
Neuroscience
Functional changes in neuropeptide Y- and somatostatin-containing neurons induced by limbic seizures in the rat
Neuroscience
Cytoskeletal dynamics in dendritic spines: direct modulation by glutamate receptors?
Trends Neurosci.
Neuropeptide Y: emerging evidence for a functional role in seizure modulation
Trends Neurosci.
Neuropeptide Y inhibits glutamate release and long-term potentiation in rat dentate gyrus
Brain Res.
Temporal and spatial appearance of the membrane cytoskeleton and perineuronal nets in the rat neocortex
Neurosci. Lett.
Calmodulin mediates calcium-dependent inactivation of N-methyl-d-aspartate receptors
Neuron
Immunostaining for substance P receptor labels GABAergic cells with distinct termination patterns in the hippocampus
J. comp. Neurol.
Neuropeptide Y distribution in the rat brain
Science
Role of actin in anchoring postsynaptic receptors in cultured hippocampal neurons: differential attachment of NMDA versus AMPA receptors
J. Neurosci.
Development of the hippocampal region in the rat. I. Neurogenesis examined with 3H-thymidine autoradiography
J. comp. Neurol.
Giant synaptic potentials in immature rat CA3 hippocampal neurones
J. Physiol.
The structure and function of alpha-actinin
J. Musc. Res. Cell Motil.
Physiological properties of anatomically identified axo-axonic cells in the rat hippocampus
J. Neurophysiol.
Synaptic connections of neuropeptide Y (NPY) immunoreactive neurons in the hilar area of the rat hippocampus
J. comp. Neurol.
Prominent expression of two forms of glutamate decarboxylase in the embryonic and early postnatal rat hippocampal formation
J. Neurosci.
The role of excitatory amino acids and NMDA receptors in traumatic brain injury
Science
Interneurons of the hippocampus
Hippocampus
Interneurons containing calretinin are specialized to control other interneurons in the rat hippocampus
J. Neurosci.
Subdivisions in the multiple GABAergic innervation of granule cells in the dentate gyrus of the rat hippocampus
Eur. J. Neurosci.
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