Functionally different AMPA-type glutamate receptors in morphologically identified neurons in rat superficial superior colliculus
Section snippets
Preparations
The present experiments were approved by the Animal Research Committee in the Okazaki National Research Institutes. All efforts were made to minimize both the suffering and the number of animals used in this study. The methods employed in this study were in general similar to those described in the preceding studies (Isa et al., 1998, Saito and Isa, 1999). Frontal slices (200–500 μm thick) of the SC were prepared from young (17–23-day-old) and adult (60–68-day-old) Wistar rats (Japan SLC,
Results
Data were obtained from 284 neurons in slices from young rats and 67 neurons in slices from adult rats. Because of difficulties in obtaining healthy slices from adult rats, most experiments were performed using young rats except where otherwise specified. The recorded neurons were distributed throughout the whole depth of the sSC.
Expression of two types of AMPA receptors in the rat superficial superior colliculus
The results of the present study have shown that both neurons with linear or outwardly rectifying KA responses and those with inwardly rectifying KA responses are present in the rat sSC. The reversal potentials of the KA responses in the isotonic Na+-free, high Ca2+ solution were inversely correlated with the RI value, indicating that neurons with stronger inward rectification showed higher Ca2+ permeability. Previous studies have demonstrated that two types of functionally different AMPA
Acknowledgements
We thank Dr. Yasuhiko Saito for valuable comments on the earlier version of the manuscript. This study was supported by grants from the Ministry of Education, Science, Culture and Sports of Japan (Projects 10480232, 10164247 and 11145241), the Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation and the Mitsubishi Foundation, Naito Memorial Foundation and Daiko Foundation.
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