Comparison of matrix metalloproteinase expression during Wallerian degeneration in the central and peripheral nervous systems
Section snippets
Wallerian degeneration
Adult Lewis rats obtained from Harlen Olac (Bicester, Oxon, UK), weighing 200–250 g at the beginning of the experiment, were used. During the study the rats were housed in cages of two or three animals and allowed access to food and water ad libitum. Animals were cared for in strict accordance with The Animals (Scientific Procedures) Act, 1986, under a Home Office license. All efforts were made to minimise both the suffering and the numbers of animals used. On day 0 the rats were anaesthetised
Results
The rate of loss of the axonal protein neurofilament-H (NF-H) and the myelin proteins, MBP and myelin-associated glycoprotein (MAG), was studied by immunohistochemistry during Wallerian degeneration in the rat sciatic and optic nerves. In corroboration with other studies (Bignami et al., 1981, Stoll et al., 1989b, George and Griffin, 1994), we found that during Wallerian degeneration in the sciatic nerve no NF-H immunostaining was detected by day 10 and that by day 15 all MBP- and MAG-positive
Discussion
In this study we have investigated the change in expression of 10 MMPs, representing each major subfamily, during the course of Wallerian degeneration in the rat nervous system. The morphological sequence of events that characterise Wallerian degeneration following nerve injury in the rat sciatic or optic nerve has been well defined; for example, see (Stoll et al., 1989a, Stoll et al., 1989b, George and Griffin, 1994, Perry et al., 1995). From this we can elucidate a potential role for the MMPs
Acknowledgements
We would like to thank the BBSRC (UK) and British Biotechnology Pharmaceuticals for support and the generous supply of excellent research tools.
References (57)
- et al.
Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence
Brain Res.
(1972) - et al.
Matrix metalloproteinases degrade myelin basic protein
Neurosci. Lett.
(1995) - et al.
Macrophage metalloelastase degrades matrix and myelin proteins and processes a tumour necrosis factor-alpha fusion protein
Biochem. Biophys. Res. Commun.
(1996) - et al.
Matrix metalloproteinase expression during experimental autoimmune encephalomyelitis and effects of a combined matrix metalloproteinase and tumour necrosis factor-alpha inhibitor
J. Neuroimmunol.
(1997) - et al.
The pattern of axonal degeneration in the peripheral nervous system varies with different types of lesion
Neuroscience
(1990) - et al.
Immunocytological localization of the major peripheral nervous system glycoprotein P0 and the L2/HNK-1 and L3 carbohydrate structures in developing and adult mouse sciatic nerve
Dev. Biol.
(1988) - et al.
Matrix metalloproteinases: they’re not just for matrix anymore!
Curr. Opin. Cell Biol.
(2001) - et al.
Macrophages and the nervous system
Int. Rev. Cytol.
(1991) - et al.
Proteolytic and non-proteolytic activation of human neutrophil progelatinase B
Biochim. Biophys. Acta
(1995) Calcium-induced degeneration of axoplasm in isolated segments of rat peripheral nerve
Brain Res.
(1974)
Lipid-protein relationships in normal and degenerating myelin
Neuropatol. Pol.
Cleavage of the P0 glycoprotein of the rat peripheral nerve myelin: tentative identification of cleavage site and evidence for the precursor-product relationship
Neurochem. Res.
Mitosis of Schwann cells and demyelination are induced by the amyloid precursor protein and other protease inhibitors in the rat sciatic nerve
Eur. J. Neurosci.
Production of plasminogen activator in cultures of superior cervical ganglia and isolated Schwann cells
Proc. Natl. Acad. Sci. USA
Cellular uptake of exogenous horseradish peroxidase in mouse peripheral nerve
Acta Neuropathol. (Berlin)
Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP
J. Neurosci. Res.
S100 is present in developing chicken neurons and Schwann cells and promotes motor neuron survival in vivo
J. Neurobiol.
Plasminogen activators in rat neural tissues during development and in Wallerian degeneration
Acta Neuropathol. (Berlin)
The fate of axonal debris in Wallerian degeneration of rat optic and sciatic nerves. Electron microscopy and immunofluorescence studies with neurofilament antisera
J. Neuropathol. Exp. Neurol.
Biochemical studies of myelin in Wallerian degeneration of rat optic nerve
J. Neurochem.
Sequential changes in the permeability of the blood-nerve barrier over the course of ricin neuronopathy in the rat
Neurotoxicology
Glial cells in transected optic nerves of immature rats. I. An analysis of individual cells by intracellular dye-injection
J. Neurocytol.
Expression of neuregulins and their putative receptors, ErbB2 and ErbB3, is induced during Wallarian degeneration
J. Neurosci.
Characterization of a neural proteinase which may play a key role in demyelinating disease
Biochem. Soc. Trans.
Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1
Immunology
The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3
Immunology
Schwann cells degrade myelin and proliferate in the absence of macrophages: evidence from in vitro studies of Wallerian degeneration
J. Neurocytol.
Cellular reactions at the lesion site after crushing of the rat optic nerve
Glia
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