Postnatal development and migration of cholecystokinin-immunoreactive interneurons in rat hippocampus
Section snippets
Immunocytochemistry
The studies were conducted in accordance with the principles and procedures outlined in the NIH Guide for the Care and Use of Laboratory Animals. All efforts were made to minimize both the suffering and the number of animals used.
Newborn (P0, n=4), 2 (P2, n=4), 4 (P4, n=3), 8 (P8, n=3), 12 (P12, n=3) and 20 (P20, n=3) day old male Wistar rats were used for the present study. Perfusion and immunostaining was performed according to the protocols described in detail earlier (Katona et al., 1999).
Results
At all ages investigated in the present study, CCK-IR cell bodies and fibers were readily seen in the strata oriens, pyramidale, radiatum, granulosum, moleculare and the hilus. In the stratum lacunosum-moleculare labeled cells were only rarely seen (Fig. 1, Fig. 2). The density of labeled cells in the hippocampus as well as their selective frequency in the different layers changed considerably during the period of postnatal development. CCK-IR processes were heavily stained and could be
Changes in the laminar distribution of CCK-IR cell bodies
The rapid decrease in the density of CCK-IR cells between P0 and P4 can be explained in several possible ways. According to published data, cells may stop synthesizing certain proteins or peptides during development, as happens with some calretinin-immunoreactive and calbindin D28k-immunoreactive cells in rat cerebral cortex. However, disappearance of these proteins from the cells in both cases takes several weeks Fonseca et al., 1995, Alcantara et al., 1996. The disappearance of neuropeptide
Acknowledgements
This work was supported by the Howard Hughes Medical Institute, the National Institutes of Health (NIH, NS 30549), and OTKA Hungary (T032251) to T.F.F. The visit of Dr. Morozov to Budapest was supported by IBRO. Monoclonal antibody 9303 raised against CCK was kindly provided by CURE/Gastroenteric Biology Center, Antibody/RIA Core, NIH grant DK 41301. The authors are grateful for the excellent technical assistance of Dr. Gábor Nyíri, Ms Katalin Lengyel and Mr. Gy Goda. Dr. Yezekiel Ben-Ari is
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