Rapid temperature changes induce adenosine-mediated depression of synaptic transmission in hippocampal slices from rats (non-hibernators) but not in slices from golden hamsters (hibernators)
Section snippets
Experimental procedures
The experiments were performed on hippocampal slices prepared from Wistar rats (n=43 slices) and golden hamsters (Mesocricetus auratus, n=21 slices). Hamsters were obtained from the Zentrale Versuchstieranstalt (Hannover, Germany). They were housed in individual cages in a long-day photoperiod (17 h light, 7 h dark) at 22±2°C (warm-acclimated hamsters). Six hamsters were transposed to a short photoperiod (7 h light, 17 h dark) and cold (7±2°C) to induce hibernation, as described previously.[17]Rats
Results
In hippocampal slices, electrical stimulation of the Schaffer collaterals/commissural fibres synaptically induces action potentials in the CA1 pyramidal cells, which can be recorded extracellularly as population action potentials (population spikes; see Fig. 2). It has been described previously that, with cooling, starting at 37°C, the population spikes induced with constant stimulus intensity first increase, reach maximal values in the temperature range of 25–30°C, then decrease with further
Discussion
To investigate disturbances in neuronal communication induced by rapid temperature changes, we studied the effects of rapid ramp-like temperature changes (0.3°C/min) on synaptic transmission in hippocampal slices prepared from golden hamsters and rats. While in hamster slices the population spike amplitude showed a temperature dependence according to previously published results,14, 16, 17, 43we found a prominent dynamic influence of the rapid temperature change in rat slices. As compared to
Acknowledgements
We thank Holger Spangenberger and Yongqiang Zhao for carrying out some experiments with adenosine application at constant temperature, and Manuel Metzner for participation in some ramp experiments. Thanks are also due to Erika Walde for excellent technical assistance, Uwe Heinemann and Holger Spangenberger for valuable discussions, and Michaela Böhm-Pinger for reading the manuscript. ω-Agatoxin IVA and CPG55845A were gifts of Pfizer (Groton, CT) and Ciba Geigy (Basel, Switzerland),
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2006, Brain ResearchCitation Excerpt :This lower sensitivity to adenosine in hamsters was proposed to be a prerequisite for safe arousal from hibernation by avoiding a temperature-induced disturbance in neuronal communication. In addition, these studies (Gabriel et al., 1998; Spangenberger et al., 1995a) showed that cellular mechanisms in the hippocampus of hibernators may be quite different than those of nonhibernators. Cellular mechanisms for several forms of neural plasticity have been intensively studied in the hippocampus of nonhibernating mammals (Bear, 2003; Kemp and Bashir, 2001; Luthi et al., 2004; Malenka and Nicoll, 1999) as they likely underlie memory and learning (Tang et al., 2001).