Cellular and subcellular localization of 5-hydroxytryptamine1B receptors in the rat central nervous system: immunocytochemical, autoradiographic and lesion studies
Section snippets
Animals
Male Wistar rats weighing 300–350 g were maintained under controlled environmental conditions (22–24°C: 12/12 h light/dark cycle, food and water ad libitum). All efforts were made to minimize animal suffering and to reduce the number of animals used.
Surgical procedures
For lesion of the striatopallidal and striatonigral pathways, rats were anaesthetized with pentobarbital (50 mg/kg, i.p.) and placed in a stereotaxic frame (David Kopf Inc.). Two injections of kainic acid (1 μg dissolved in 1 μl of 0.9% NaCl at each
Results
Extensive characterization of the antibodies raised against the selective Val263–Lys287 portion of the rat 5-HT1B receptor protein, which were used in the present study, has clearly demonstrated their specificity for this receptor.34, 52
Distribution of 5-hydroxytryptamine1B receptor and binding sites in the rat brain
Comparison of the distribution of 5-HT1B receptor-like immunoreactivity in the rat brain with maps derived from the autoradiographic detection of 5-HT1B binding sites using [125I]CYP or serotonin-O-carboxymethylglycyl-[125I]iodotyrosinamide ([125I]GTI)8, 12, 13, 47reveals a remarkable degree of similarity. High densities of both receptor-like immunoreactivity and specific binding sites are present in globus pallidus, ventral pallidum, substantia nigra and dorsal subiculum.8, 12, 34, 52Other
Conclusion
A remarkable similarity exists between the distribution of 5-HT1B receptors established from immunohistochemical and radioligand binding studies.8, 12, 13, 34, 47, 52The high resolution power of immunocytochemical techniques allowed the subcellular visualization of 5-HT1B receptors. At the ultrastructural level, we found that the protein is essentially located on axons and axon terminals in the globus pallidus, the substantia nigra and the superficial gray layer of the superior colliculus. In
Acknowledgements
This work was supported by grants from CNRS, INSERM, DRET (Contract n° 95-142), the MERT (Ministère de l'Education et de la Recherche et de la Technologie), the EEC (Biotech, contract BI04-CT96-0752), and the ECOS Committee (Action n° C94-B03). We are grateful to Mr P. Nguyen for his excellent photographic work.
References (65)
- et al.
Serotonin1B receptor regulation after dorsal subiculum deafferentation
Brain Res. Bull.
(1995) - et al.
Activation of 5-HT1B receptors suppresses low but not high frequency synaptic transmission in the rat subicular cortex in vitro
Brain Res.
(1996) - et al.
Molecular biology of 5-HT receptors
Neuropharmacology
(1994) - et al.
The mouse 5-hydroxytryptamine1B receptor is localized predominantly on axon terminals
Neuroscience
(1994) - et al.
Effects of retinal deafferentation on serotonin receptor types in the superficial grey layer of the superior colliculus of the rat
J. chem. Neuroanat.
(1993) - et al.
Distractibility and locomotor activity in rat following intra-collicular injection of a serotonin1B–1D agonist
Behav. Brain Res.
(1995) - et al.
Localization of 5-HT1B, 5-HT1Dα, 5-HT1E and 5-HT1F receptor messenger RNA in rodent and primate brain
Neuropharmacology
(1994) - et al.
Downregulation of muscarinic- and 5-HT1B-mediated modulation of [3H]acetylcholine release in hippocampal slices of rats with fimbria–fornix lesions and intrahippocampal grafts of septal origin
Brain Res.
(1995) - et al.
Ultrastructure and synaptic targets of the raphe-nigral projection in the rat
Neuroscience
(1993) - et al.
Stimulation of 5-HT1A and 5-HT1B receptors in brain regions and its effects on male rat sexual behaviour
Eur. J. Pharmac.
(1992)
Molecular cloning and functional characterization of a human 5-HT1B serotonin receptor: a homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity
Biochem. biophys. Res. Commun.
Alignment of receptor nomenclature with the human genome: classification of 5-HT1B and 5-HT1D receptor subtypes
Trends pharmac. Sci.
Of mice and flies: commonalities among 5-HT receptors
Trends pharmac. Sci.
5-HT receptor classification and nomenclature: towards a harmonization with the human genome
Neuropharmacology
Characterization of the human 5-hydroxytryptamine1B receptor
J. biol. Chem.
5-HT1B agonists induce anorexia at a postsynaptic site
Eur. J. Pharmac.
Differential addressing of 5-HT1A and 5-HT1B receptors in transfected LLC-PK1 epithelial cells: a model of receptor targeting in neurons
Neuroscience
Cholinergic terminals in rat hippocampus possess 5-HT1B receptors mediating inhibition of acetylcholine release
Eur. J. Pharmac.
Conversion of the human 5-HT1Dβ serotonin receptor to the rat 5-HT1B ligand-binding phenotype by Thr355Asn site directed mutagenesis
Biochem. Pharmac.
Potentiation of fluoxetine-induced penile erections by combined blockade of 5-HT1A and 5-HT1B receptors
Eur. J. Pharmac.
Serotonin modulates retinotectal and corticotectal convergence in the superior colliculus
Prog. Brain Res.
Quantitative and morphometric data indicate precise cellular interactions between serotonin terminals and postsynaptic targets in rat substantia nigra
Neuroscience
β-adrenoceptor blocking agents recognize a subpopulation of serotonin receptors in brain
Brain Res.
Light and electron microscopic immunocytochemical visualization of 5-HT1B receptors in the rat brain
Brain Res.
Striatal lesions with kainic acid: neurochemical characteristics
Brain Res.
Do 5-HT1B/1D autoreceptors modulate dorsal raphe firing? In vivo electrophysiological studies in guinea pig with GR 127935
Neuropharmacology
Serotonin-1 receptor binding sites in the human basal ganglia are decreased in Huntington's chorea but not in Parkinson's disease: a quantitative in vitro autoradiography study
Neuroscience
5-HT1D receptors in the guinea pig brain: pre- and postsynaptic localizations in the striatonigral pathway
Brain Res.
The rat 5-hydroxytryptamine1B receptor is the species homologue of the human 5-hydroxytryptamine1Dβ receptor
Molec. Pharmac.
Sumatriptan inhibits the release of CGRP and substance P from the rat spinal cord
NeuroReport
Detailed mapping of serotonin 5-HT1B and 5-HT1D receptor messenger RNA and ligand binding sites in guinea-pig brain and trigeminal ganglion: clues for function
Neuroscience
Biochemical and pharmacological characterization of serotonin-O-carboxymethylglycyl-[125I]iodotyrosinamide, a new radioiodinated probe for 5-HT1B and 5-HT1D binding sites
J. Neurochem.
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