Immunity
Volume 7, Issue 1, 1 July 1997, Pages 25-36
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Article
EBF and E47 Collaborate to Induce Expression of the Endogenous Immunoglobulin Surrogate Light Chain Genes

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Abstract

Early B cell factor (EBF) and E47 participate in the transcriptional control of early B lymphocyte differentiation. With the aim of identifying genetic targets for these transcription factors, we stably transfected cDNAs encoding EBF or a covalent homodimer of E47, individually or together, into immature hematopoietic Ba/F3 cells, which lack both factors. In combination, EBF and E47 induce efficient expression of the endogenous immunoglobulin surrogate light chain genes, λ5 and VpreB, whereas other pre–B cell–specific genes remain silent. Multiple functionally important EBF and E47 binding sites were identified in the λ5 promoter/enhancer region, indicating that λ5 is a direct genetic target for these transcription factors. Taken together, these data suggest that EBF and E47 synergize to activate expression of a subset of genes that define an early stage of the B cell lineage.

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