Math1-driven GFP expression in the developing nervous system of transgenic mice
Section snippets
Results and discussion
Math1 is a basic helix–loop–helix (bHLH) transcription factor that is expressed in and/or required for the development of specific cell populations in the nervous system including cerebellar granule cells (Ben-Arie et al., 1997), hair cells of the inner ear (Bermingham et al., 1999, Chen et al., 2002), commissural interneurons of the dorsal spinal cord identified by the lim homeodomain factors Lhx2/9 (Bermingham et al., 2001, Gowan et al., 2001), and Merkel cells in the epidermis (Ben-Arie et
Generation of transgenic mice
A 1,385-bp sequence found ∼3 kb 3′ of the Math1 coding sequence (nt 1-1385 from AF218258) was placed 5′ of the BgnGFP reporter construct. The BgnGFP reporter construct contains the β-globin basal promoter (from BGZA, (Yee and Rigby, 1993)), the Nco1/Xho1 sequence from Clontech pIRES-EGFP containing EGFP with bovine growth hormone 3′ sequences, and an added nuclear localization signal at the N-terminus (MAPKKKRKVDEV). The Math1 enhancer contains the same sequence as Tg15 in (Helms et al., 2000)
Acknowledgements
We gratefully acknowledge Drs K. Lee and T. M. Jessell for providing LH2A/B antisera, and the Developmental Studies Hybridoma Bank for TAG-1 specific antibodies (4D7). E. A. L. is supported by the Sandler Program in Basic Sciences. H. H. is a NSF predoctoral fellow. P. C. is supported by the NIH. A. D., P. W., A. G. and N. S. are supported by the NIH and the National Organization for Hearing Research. T. C, P. P., and A. O.-A. are supported by grants from the March of Dimes and the Texas
References (30)
- et al.
Specific subtypes of cutaneous mechanoreceptors require Neurotrophin-3 following peripheral target innervation
Neuron
(1996) - et al.
A mammalian helix–loop–helix factor structurally related to the product of the Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system
J. Biol. Chem.
(1995) - et al.
Proprioceptor pathway development is dependent on MATH1
Neuron
(2001) - et al.
Spatial regulation of axonal glycoprotein expression of subsets of embryonic spinal neurons
Neuron
(1988) - et al.
The axonal glycoprotein TAG-1 is an immunoglobulin superfamily member with neurite outgrowth-promoting activity
Cell
(1990) - et al.
Crossinhibitory activities of Ngn1 and Math1 allow specification of distinct dorsal interneurons
Neuron
(2001) - et al.
Overexpression of MATH1 disrupts the coordination of neural differentiation in cerebellum development
Mol. Cell. Neurosci.
(2001) - et al.
Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm
Cell
(1995) - et al.
A fluorescent microscopic study of the development of rat touch domes and their Merkel cells
Neuroscience
(1984) - et al.
Relationship between Merkel cells and nerve endings during embryogenesis in the mouse epidermis
J. Invest. Dermatol.
(1990)
Lineage-specific regulation of the neural differentiation gene MASH1
Dev. Biol.
Generation of cerebellar granule neurons in vivo by transplantation of BMP-treated neural progenitor cells
Nature Neurosci.
Math1 is essential for genesis of cerebellar granule neurons
Nature
Functional conservation of atonal and Math1 in the CNS and PNS
Development
Math1: an essential gene for the generation of inner ear hair cells
Science
Cited by (244)
Precise detection of CRISPR-Cas9 editing in hair cells in the treatment of autosomal dominant hearing loss
2022, Molecular Therapy Nucleic AcidsCitation Excerpt :Kharkovet et al. generated a knock-out mouse model with a deletion of Kcnq4 exons 6–8, and no hearing impairment was observed in Kcnq4+/− animals, which suggests that the disruption of KCNQ4 mutant allele is a potential strategy to treat hearing loss caused by KCNQ4 heterozygous pathogenic variants.31 The hair cells express GFP specifically in the Atoh1-GFP mice, where a portion of the Atoh1 enhancer sequence was randomly integrated into the mouse genome, directing the expression of the reporter gene GFP.33 It suggests the Atoh1-GFP mice could be utilized to precisely detect the editing efficiency of CRISPR-Cas9 technology in vivo.
A Low Dose of Rapamycin Promotes Hair Cell Differentiation by Enriching SOX2<sup>+</sup> Progenitors in the Neonatal Mouse Inner Ear Organoids
2024, JARO - Journal of the Association for Research in Otolaryngology