Biochemical and Biophysical Research Communications
Involvement of the calcium channel β3 subunit in olfactory signal transduction
Section snippets
Methods
The β3-deficient mouse (β3−/−) was constructed as previously reported and back-crossed for more than 20 generations [10]. The wild-type (β3+/+) and β3-deficient (β3−/−) mice were 12- to 16-week-old, experimentally naive mice. The animals were maintained at 22 ± 0.5 °C under a 12-h light–dark cycle. All experiments were conducted during the light phase of the cycle and carried out in accordance with the Guidelines for the Use of Laboratory Animals of the Graduate School of Pharmaceutical Sciences,
RNA transcription
In analyzing the RNA transcripts of the α1 and β subunits, 204-, 273-, 234-, 242-, 417-, 249-, 219-, 187-, and 195-bp fragments corresponding to the CaV2.1 (α1A), CaV2.2 (α1B), CaV1.2 (α1C), CaV1.3 (α1D), CaV2.3 (α1E), β1, β2, β3, and β4 subunit-specific fragments, respectively, were generated by PCR (Fig. 1A). Because CaV2.1, CaV2.2, and CaV2.3 are thought to compose the P/Q-, N-, and R-type Ca2+ channels, our results indicate the presence of these Ca2+ channels in olfactory tissue and suggest
Acknowledgments
We thank Drs. Veit Flockerzi, David Stevens, and Bernd Lindemann for their help.
References (18)
Dialysis of inositol 1,4,5-trisphosphate induces inward currents and Ca2+ uptake in frog olfactory receptor cells
Biochem. Biophys. Res. Commun.
(1996)Signaling pathways in odorant detection
Neuron
(1992)- et al.
Localization of mRNA of voltage-dependent Ca2+-channels: four subtypes of α1- and β-subunits in developing and mature rat brain
Mol. Brain Res.
(1995) - et al.
Pain perception in mice lacking the β3 subunit of voltage-activated calcium channels
J. Biol. Chem.
(2002) - et al.
Modified cardiovascular L-type channels in mice lacking the voltage-dependent Ca2+ channel β3 subunit
J. Biol. Chem.
(2003) - et al.
Protease sensitive urinary pheromones induced region-specific Fos-expression in rat accessory olfactory bulb
Biochem. Biophys. Res. Commun.
(1999) - et al.
The odorant-sensitive adenylate cyclase of olfactory receptor cells: differential stimulation by distinct classes of odorants
J. Biol. Chem.
(1986) - et al.
Odorants of the same odor class activate different second messenger pathways
Chem. Senses
(1991) - et al.
Transduction and adaptation in sensory receptor cells
J. Neurosci.
(1995)
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