Biochemical and Biophysical Research Communications
Trb2, a mouse homolog of tribbles, is dispensable for kidney and mouse development
Section snippets
Materials and methods
Generation of Trb2 mutant mice. The homologous arms of the targeting vector were isolated using the Red/ET recombination cloning system (Gene Bridges GmbH) and a bacterial artificial chromosome (BAC) clone obtained from the C57BL/6 library. The 5′arm (8.3 kb) was obtained using the primers 5′-AACCCAGCCAGCAGCTCCAGAATT-3′ and 5′-GCTCATCCATCTCTCCAGAGGGGTT-3′, while the 3′ arm (4.1 kb) was obtained using 5′-AGACTCCCGGGATCATTATGCGTG-3′ and 5′-TCTGCCTGTCTGCCCATAGCAACAT-3′. The 5′ arm was cloned into
Generation of Trb2 mutant mice
To examine the roles of Trb2 in vivo, we generated Trb2 mutant mice using embryonic stem cells. The Trb2 gene is composed of three exons, and exon 1 includes the first ATG site. We replaced a portion of exon 1 including 44 base pairs upstream of the initiation codon with the LacZ-Neo cassette, which consists of a β-galactosidase gene and a neomycin-resistance gene sandwiched by lox71 and loxP sequences (Fig. 1A). Southern blotting analysis and genomic PCR confirmed correct homologous
Discussion
Trb2 is a mouse homolog of Drosophila tribbles. By generating Trb2-lacZ mutant mice, we showed that Trb2 is expressed in the podocytes of developing kidney glomeruli, as well as in many other tissues. However, homozygous mice did not exhibit any apparent phenotypes. Glomeruli were formed and no proteinuria was observed, suggesting normal podocyte functions in the absence of Trb2. Our data indicate that Trb2 has minimal functions in kidney and mouse development.
We detected Trb2 expression in a
Acknowledgments
We thank H. Kobayashi for helpful discussions. This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology and by the Ministry of Health, Labor and Welfare of Japan. Minoru Takasato was supported by the Japan Society for the Promotion of Science.
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