Archival ReportLate Adolescent Expression of GluN2B Transmission in the Prefrontal Cortex Is Input-Specific and Requires Postsynaptic Protein Kinase A and D1 Dopamine Receptor Signaling
Section snippets
Methods and Materials
All experimental procedures were approved by the Rosalind Franklin University Institutional Animal Care and Use Committee according to the U.S. Public Health Service Guide for Care and Use of Laboratory Animals. Male Sprague-Dawley rats (Harlan, Indianapolis, Indiana) from P25 to P85 were allowed to acclimate to the animal facility for at least 5 days before being used, group-housed (2 to 3 rats per cage) with food and water available ad libitum, and maintained at 21°C to 23°C in a 12-hour
Results
We first examined whether glutamatergic transmission onto the apical dendrite of layer V medial PFC pyramidal neurons is developmentally regulated during the transition from juvenile/early adolescence (P25–P40) to late adolescence/adulthood (P50–P80). Analyses of the evoked response revealed that both age groups exhibit similar EPSC amplitude at the −70 mV (EPSC−70mV) and at the +60 mV (EPSC+60mV) holding potentials (Figure 1A). Similarly, the EPSC−70mV duration was indistinguishable among
Discussion
In the present study, we found that NMDA-mediated transmission onto the apical dendrite of layer V pyramidal neurons in the medial PFC undergoes a specific developmental upregulation during the adolescent transition to adulthood. Both postsynaptic PKA signaling and local prefrontal D1 receptor tone are necessary, but not sufficient, to sustain the characteristic long-lasting NMDA response that begins to emerge ~P45 (Figure 8A,B). Our results also indicate that such developmental facilitation is
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2021, NeuronCitation Excerpt :For example, experience-dependent synaptic pruning is regulated by glucocorticoid stress hormones, yet prolonged glucocorticoid exposure during development can cause excessive and irreversible synapse loss (Liston and Gan, 2011; Liston et al., 2013). Finally, as demonstrated by rodent studies, association regions also display an increase in NMDA NR2B-dependent neurotransmission during the peripubertal (quasi-adolescent) period, which facilitates continued plasticity within higher-order cortex (Flores-Barrera et al., 2014). Microscale studies of cortical excitation thus corroborate the prolonged period of association cortex maturation identified by neuroimaging data and reveal that excitatory plasticity-related events may be important drivers of how structural and functional imaging measures change during hierarchical neurodevelopment.