Cell
Volume 163, Issue 2, 8 October 2015, Pages 456-492
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Reconstruction and Simulation of Neocortical Microcircuitry

https://doi.org/10.1016/j.cell.2015.09.029Get rights and content
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Highlights

  • The Blue Brain Project digitally reconstructs and simulates a part of neocortex

  • Interdependencies allow dense in silico reconstruction from sparse experimental data

  • Simulations reproduce in vitro and in vivo experiments without parameter tuning

  • The neocortex reconfigures to support diverse information processing strategies

Summary

We present a first-draft digital reconstruction of the microcircuitry of somatosensory cortex of juvenile rat. The reconstruction uses cellular and synaptic organizing principles to algorithmically reconstruct detailed anatomy and physiology from sparse experimental data. An objective anatomical method defines a neocortical volume of 0.29 ± 0.01 mm3 containing ∼31,000 neurons, and patch-clamp studies identify 55 layer-specific morphological and 207 morpho-electrical neuron subtypes. When digitally reconstructed neurons are positioned in the volume and synapse formation is restricted to biological bouton densities and numbers of synapses per connection, their overlapping arbors form ∼8 million connections with ∼37 million synapses. Simulations reproduce an array of in vitro and in vivo experiments without parameter tuning. Additionally, we find a spectrum of network states with a sharp transition from synchronous to asynchronous activity, modulated by physiological mechanisms. The spectrum of network states, dynamically reconfigured around this transition, supports diverse information processing strategies.

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