Developmental Cell
Volume 29, Issue 5, 9 June 2014, Pages 577-590
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Article
LC3 Binding to the Scaffolding Protein JIP1 Regulates Processive Dynein-Driven Transport of Autophagosomes

https://doi.org/10.1016/j.devcel.2014.04.015Get rights and content
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Highlights

  • Retrograde axonal transport of autophagosomes requires the scaffolding protein JIP1

  • JIP1 binds directly to the autophagosome protein LC3 via a conserved LIR motif

  • LC3-binding to JIP1 blocks the JIP1-mediated activation of kinesin-1 motor activity

  • JIP1 dephosphorylation by organelle-bound MKP1 may also block kinesin-1 activation

Summary

Autophagy is essential for maintaining cellular homeostasis in neurons, where autophagosomes undergo robust unidirectional retrograde transport along axons. We find that the motor scaffolding protein JIP1 binds directly to the autophagosome adaptor LC3 via a conserved LIR motif. This interaction is required for the initial exit of autophagosomes from the distal axon, for sustained retrograde transport along the midaxon, and for autophagosomal maturation in the proximal axon. JIP1 binds directly to the dynein activator dynactin but also binds to and activates kinesin-1 in a phosphorylation-dependent manner. Following JIP1 depletion, phosphodeficient JIP1-S421A rescues retrograde transport, while phosphomimetic JIP1-S421D aberrantly activates anterograde transport. During normal autophagosome transport, residue S421 of JIP1 may be maintained in a dephosphorylated state by autophagosome-associated MKP1 phosphatase. Moreover, binding of LC3 to JIP1 competitively disrupts JIP1-mediated activation of kinesin. Thus, dual mechanisms prevent aberrant activation of kinesin to ensure robust retrograde transport of autophagosomes along the axon.

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