Identification and comparative expression analyses of Daam genes in mouse and Xenopus
Section snippets
Results and discussion
Wnts are key orchestrators of cell fate specification and the morphogenetic movements that occur during vertebrate axis formation and gastrulation (reviewed in Yamaguchi, 2001, Tada et al., 2002, Myers et al., 2002, Veeman et al., 2003). Studies in zebrafish and Xenopus indicate that Wnts control vertebrate gastrulation movements by signaling through non-canonical, βcatenin-independent, signaling pathways. Several non-canonical pathways have been proposed, including the Wnt/Ca2+, the Wnt/JNK,
Isolation of mDaam1 and mDaam2 cDNAs
To isolate mDaam1 and mDaam2 cDNAs, we performed a PCR-based screen of an E12.5 Swiss Webster embryo cDNA library using Rapid-Screen™ Arrayed cDNA Library Panels (Origene Tecnologies Inc. MEB-1001). Primer pairs used for mDaam1 cDNA screening were based on sequences derived from ESTs (see below):
forward primer 5′-GTAACCAGATTCATCGACTT-3′
reverse primer 5′-CTTGCAGAACTTTCTTGTGG-3′
Similarly, primer pairs used for mDaam2 cDNA screening are as follows:
forward primer 5′-TGTCAATGTGGACCATGTCA-3′,
reverse
Acknowledgements
We thank Mark Lewandoski, Rivka Rachel and lab members for discussion and comments on the manuscript. RH is supported by an IRTA fellowship (NICHD/NIH) and gratefully acknowledges the support and guidance of Igor B. Dawid.
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