Memory consolidation and reconsolidation of an inhibitory avoidance response in mice: effects of i.c.v. injections of hemicholinium-3
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Experimental subjects
CF-1 male mice (FUNDACAL, Buenos Aires, Argentina) were used (age: 60–70 days; weight: 25–30 g). They were individually caged and remained singly housed throughout the experimental procedures. The mice were kept in a climatised animal room (21–23 °C) maintained on a 12-h light/dark cycle (lights on at 06:00 h), with ad libitum access to dry food and tap water. Experiments were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH
Effects of post-training i.c.v. of HC-3 on retention
The results are shown in Fig. 1. The post-training i.c.v. administration of HC-3 impaired retention performance in mice that received the footshock during the learning trial. The effect was observed not only 48 h after training, but also 7 days after it (P<0.01, in both cases as compared with the respective saline injected control group). There was not significant difference in the response latencies among the groups that were trained without footshock but were injected with saline or HC-3
Discussion
The results of the first experiment indicate that post-training i.c.v. administration of HC-3, a selective inhibitor for the function of cholinergic neurons (Gardiner, 1961, MacIntosh, 1963), impairs retention performance of a one-trial inhibitory avoidance response in mice. The effect was observed at each training-test interval (i.e. 48 h or 7 days) at which the mice were tested once, and only in those mice that had received a footshock on the learning trial. Thus, the post-training effects of
Acknowledgements
This work was supported by grant B035 from the University of Buenos Aires and M. G. Blake is a fellow from UBA.
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