Clinical neuroscienceSerial analysis of gene expression in the hippocampus of patients with mesial temporal lobe epilepsy
Section snippets
Tissue
In order to make a sensible comparison between control tissue and surgical tissue from patients we needed material from controls with the shortest postmortem interval possible. For obvious reasons this material is very rarely available and therefore we used only one control specimen. Control hippocampus (used to construct the SAGE control library) was obtained from a 48 year old man without history of seizures or other neurological diseases and no brain abnormalities at autopsy and
Results
The main aim of this study was to construct and compare SAGE libraries of control human hippocampus and hippocampus of patients with MTLE and HS.
Discussion
In this study we used SAGE to provide information on the human hippocampal transcriptome. As SAGE is an open system-based gene screening procedure, it provides an unbiased view of the transcriptome, capable of identifying both known and novel hippocampal genes and their relative expression levels under normal and epileptic conditions.
The database generated by this study represents an extensive inventory of approximately 9000 different genes that are most abundantly expressed in the human
Conclusion
In conclusion, identification and assessment of the gene expression profile is a useful first step in understanding molecular mechanisms leading to or representing the neuropathology of HS. The database generated by this study provides an extensive inventory of genes expressed in human control hippocampus, identifies new high-abundant genes associated with altered hippocampal morphology in patients with MTLE and serves as a reference for future studies aimed at detecting hippocampal
Acknowledgments
The work was supported by the National Epilepsy Fund “Power of the Small” and Hersenstichting Nederland (NEF grant 02–10 and 05–11; E. Aronica and K. Boer), the “Christelijke Vereniging voor de Verpleging van Lijders aan Epilepsie” (E. Aronica) and by Hacettepe University Research Fund (project number: 01 02 101 007; M. Özgüç). Filiz Ozbas-Gerceker was supported by European Science Foundation–Integrated Approaches for Functional Genomics Programme Exchange grant. We express gratitude to Prof.
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2013, Journal of Clinical NeuroscienceCitation Excerpt :A significant relationship between complicated febrile seizures (FS) and MTS has been reported.4,5 A number of molecular studies on human brain tissue in MTS have been performed6,7 with many gene expression studies carried out in animal models and using human biopsy specimens.8,9 However, the mechanism and genetic background of this unique pathology is currently not elucidated.
- 1
The first two authors contributed equally to the present work.