Elsevier

Vision Research

Volume 45, Issue 28, December 2005, Pages 3454-3468
Vision Research

Excitation mapping with the organic cation AGB2+

https://doi.org/10.1016/j.visres.2005.07.025Get rights and content
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Abstract

Excitation mapping is a method of visualizing the signaling history of neurons with permeant organic cations. It is compatible with high-resolution imaging, allowing concurrent visualization of all neuronal classes and their glutamate-gated excitation histories. Excitation mapping documents the stability and precision of neuronal signaling within a given neuronal class, arguing that single unit electrophysiological sampling accurately reflects neuronal diversity. We here review the theory of excitation mapping, provide methods and protocol links; outline imaging concepts; provide parametric data on the temporal range and physiological sensitivity of excitation mapping; and show that immunocytochemical methods for macromolecules are compatible with excitation mapping.

Keywords

Glutamate receptors
Immunocytochemistry
1-Amino-4-guanidobutane
Agmatine
Imaging
Excitation mapping

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