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Myelinating Schwann cells determine the internodal localization of Kv1.1, Kv1.2, Kvβ2, and Caspr

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Journal of Neurocytology

Abstract

We examined the localization of Caspr and the K+ channels Kv1.1 and Kv1.2, all of which are intrinsic membrane proteins of myelinated axons in the PNS. Caspr is localized to the paranode; Kv1.1, Kv1.2 and their β2 subunit are localized to the juxtaparanode. Throughout the internodal region, a strand of Caspr staining is flanked by a double strand of Kv1.1/Kv1.2/Kvβ2 staining. This tripartite strand apposes the inner mesaxon of the myelin sheath, and forms a circumferential ring that apposes the innermost aspect of Schmidt-Lanterman incisures. The localization of Caspr and Kv1.2 are not disrupted in mice with null mutations of the myelin associated glycoprotein, connexin32, or Kv1.1 genes. At all of these locations, Caspr and Kv1.1/Kv1.2/Kvβ2 define distinct but interrelated domains of the axonal membrane that appear to be organized by the myelin sheath.

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Authors and Affiliations

  1. Department of Neurology, The University of Pennsylvania Medical Center, USA

    Edgardo J. Arroyo & Yi-Tian Xu

  2. Dept. of Physiology, The University of Wisconsin, 1300 University Ave., Room 285 Medical Science Bldg., Madison, WI, 53706, USA

    Lei Zhou & Shing Yan Chiu

  3. Department of Pathobiological Sciences, School of Veterinary Medicine, The University of Wisconsin-Madison, Madison, WI, 53706, USA

    Albee Messing

  4. Department of Molecular Cell Biology, Weizmann Institute of Science, 76100, Rehovot, Israel

    Elior Peles

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  1. Edgardo J. Arroyo
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  2. Yi-Tian Xu
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  3. Lei Zhou
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  5. Elior Peles
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  6. Shing Yan Chiu
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Arroyo, E.J., Xu, YT., Zhou, L. et al. Myelinating Schwann cells determine the internodal localization of Kv1.1, Kv1.2, Kvβ2, and Caspr. J Neurocytol 28, 333–347 (1999). https://doi.org/10.1023/A:1007009613484

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