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Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1

Nature volume 306, pages 376–378 (1983)Cite this article

Abstract

Myelin-associated glycoprotein (MAG) is a quantitatively minor component in both peripheral and central myelin sheaths that is thought to have a role in cell–cell interactions within the nervous system1. We show here that a mouse monoclonal antibody, HNK-1, which is directed against human natural killer cells also recognizes an antigenic determinant of human central and peripheral nervous system white matter by immunoperoxidase staining of tissue sections. Immunoblot analysis of myelin proteins and purified extracted MAG indicates that the antigen recognized is MAG.

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References

  1. Quarles, R. H., Everley, J. L. & Brady, R. O. J. Neurochem. 21, 1171–1191 (1973).

    Article  Google Scholar 

  2. Abo, T. & Balch, C. M. J. Immun. 127, 1024–1029 (1981).

    CAS  Google Scholar 

  3. Abo, T., Cooper, M. D. & Balch, C. M. J. exp. Med. 155, 321–326 (1982).

    Article  CAS  Google Scholar 

  4. Hrapchak, B. B. in Theory and Practice of Histotechnology (eds Sheehan, D. C. & Hrapchak, B. B.) 310–326 (Mosby, St Louis, 1980).

    Google Scholar 

  5. Norton, W. T. & Poduslo, S. E. J. Neurochem. 21, 749–758 (1973).

    Article  CAS  Google Scholar 

  6. Benjamins, J. A. & Morell, P. Neurochem. Res. 3, 137–174 (1978).

    Article  CAS  Google Scholar 

  7. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  8. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  9. Quarles, R. H. & Pasnak, C. L. Biochem. J. 163, 635–637 (1977).

    Article  CAS  Google Scholar 

  10. Quarles, R. H., Barbarash, G. R., Figlewicz, D. A. & Mclntyre, L. J. Biochim. biophys. Acta. 757, 140–143 (1983).

    Article  CAS  Google Scholar 

  11. Dobersen, M. J., MacIntosh, T. D., Quarles, R. H. & Brady, R. O. Trans. int. Soc. Neurochem. (in the press).

  12. Quarles, R. H., Johnson, D., Brady, R. O. & Sternberger, N. H. Neurochem. Res. 6, 1115–1127 (1981).

    Article  CAS  Google Scholar 

  13. Sato, S., Quarles, R. H. & Brady, R. O. J. Neurochem. 39, 97–105 (1982).

    Article  CAS  Google Scholar 

  14. Kubagawa, H., Abo, T., Balch, C. M. & Cooper, M. S. Fedn Proc. 42, 1219 (1983).

    Google Scholar 

  15. Oger, J., Szuchet, S., Antel, J. & Arnason, B. G. W. Nature 295, 66–68 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Garson, J. A., Beverley, P. C. L., Coakham, H. B. & Harper, E. I. Nature 298, 375–377 (1982).

    Article  ADS  CAS  Google Scholar 

  17. Hirayama, M., Lisak, R. P., Kim, S. U., Pleasure, D. E. & Silberberg, D. Nature 301, 152–154 (1983).

    Article  ADS  CAS  Google Scholar 

  18. Burrows, P. D., Beck, G. B. & Wabl, M. R. Proc. natn. Acad. Sci. U.S.A. 78, 564–568 (1981).

    Article  ADS  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, K7L 3N6, Canada

    R. C. McGarry & J. C Roder

  2. Department of Biological Sciences, Stanford University, Stanford, California, 94305, USA

    S. L. Helfand

  3. Developmental and Metabolic Neurology Branch, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20014, USA

    R. H. Quarles

Authors
  1. R. C. McGarry
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  2. S. L. Helfand
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  3. R. H. Quarles
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  4. J. C Roder
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McGarry, R., Helfand, S., Quarles, R. et al. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature 306, 376–378 (1983). https://doi.org/10.1038/306376a0

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