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Transpostion of the Drosophila element mariner into the chicken germ line

An Erratum to this article was published on 01 January 1999

Abstract

The ability of the Drosophila transposable element mariner to transpose in the chicken was tested using a plasmid carrying an active mariner element injected into chick zygotes. Surviving embryos and chicks were analyzed for presence of mariner. Analysis of embryos that survived for at least 12 days of development indicated that mariner had transposed at high frequency into the chicken genome. Germline transmission of mariner from one of three surviving birds confirmed transposition. Analysis of the first-generation (G1) chicks showed that they each contained between one and three copies of mariner. Six different transposition events were represented in the G1 birds, and the transposition was catalyzed by expression of the mariner element's transposase gene. Transmission from G1 to G2 occurred at a 1:1 ratio. Mariner therefore has potential for development as a vector for transgenesis in avian species.

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Figure 1: Diagram of pMos1.
Figure 2: Southern blot analysis of genomic DNA isolated from individual G1 transgenic chicks, hybridized with a mariner probe.
Figure 3: Characterization of a single integrated mariner element.
Figure 4
Figure 5: Southern blot analysis of genomic DNA isolated from individual G2 transgenic embryos, digested with BamHI plus HindII and hybridized with a mariner probe.

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Acknowledgements

This work was funded by the BBSRC, in part through the ROPA scheme (H.S.), and by a project grant (042192/Z/94/Z) from the Wellcome Trust (D.F.). M. Hutchison provided excellent technical support.

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Division of Development and Reproduction, Roslin Institute, Roslin, Midlothian EH25 9PS, UK.

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Sherman, A., Dawson, A., Mather, C. et al. Transpostion of the Drosophila element mariner into the chicken germ line. Nat Biotechnol 16, 1050–1053 (1998). https://doi.org/10.1038/3497

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