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Isozyme-selective stimulation of phospholipase C-β2 by G protein βγ-subunits

Nature volume 360, pages 684–686 (1992)Cite this article

Abstract

HYDROLYSIS by phospholipase C (PLC) of phosphatidylinositol 4,5-bisphosphate is a key mechanism by which many extracellular signalling molecules regulate functions of their target cells1,2. At least eight distinct isozymes of PLC are recognized in mammalian cells3,4. Receptor-controlled PLC is often regulated by G proteins, which can be modified by pertussis toxin in some cells but not in others5 6. In the latter cells, PLC-β1, but not PLC-γl or PLC-δ1, may be activated by members of the αq-subfamily of the G protein α-subunits7–10. An unidentified PLC in soluble fractions of cultured human HL-60 granulocytes is specifically stimulated by G protein βγ subunits purified from retina and brain11. Identification of a second PLC-β complementary DNA (PLC-β2) in an HL-60 cell cDNA library9 prompted us to investigate the effect of purified G protein βγ subunits on the activities of PLC-β1 and PLC-β2 transiently expressed in cultured mammalian cells. We report here that PLC-β1 and PLC-β2 were stimulated by free βγ subunits and that PLC-β2 was the most sensitive to βγ stimulation. Thus stimulation of PLC by βγ subunits is isozyme-selective and PLC-β2 is a prime target of βγ stimulation. Activation of PLC-β2 by βγ subunits may be an important mechanism by which pertussis toxin-sensitive G proteins stimulate PLC.

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Authors and Affiliations

  1. Molecular Pharmacology Division, German Cancer Research Center, 6900, Heidelberg, Germany

    Montserrat Camps, Petra Schnabel, Alexander Scheer & Peter Gierschik

  2. Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, WC2A 3PX, UK

    Amanda Carozzi & Peter J. Parker

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  1. Montserrat Camps
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  2. Amanda Carozzi
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  3. Petra Schnabel
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  4. Alexander Scheer
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  5. Peter J. Parker
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  6. Peter Gierschik
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Camps, M., Carozzi, A., Schnabel, P. et al. Isozyme-selective stimulation of phospholipase C-β2 by G protein βγ-subunits. Nature 360, 684–686 (1992). https://doi.org/10.1038/360684a0

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