Abstract
THE eukaryotic cell cycle is regulated by the sequential activation of cyclin-dependent kinases (CDKs)1. CDK activation is dependent on cyclin binding2–4 and phosphorylation of a conserved threonine (T161 in Cdc2)5–9 mediated by the CDK-activating kinase CAK9. A CDK-related kinase, MO15 (ref. 10), has been identified as the catalytic subunit of CAK (refs 11–13). Here we use a yeast two-hybrid screen to show that a new human cyclin (cyclin H) is a MO15-associated protein. Cyclin H is a major MO15 partner in vivo and enhances the kinase activity of MO15 towards Cdk2/cyclin A. These findings demonstrate that a cyclin/kinase complex can function as a regulator of other cyclin/kinase complexes, and suggest that cyclin/kinase cascades may exist.
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Mäkelä, T., Tassan, JP., Nigg, E. et al. A cyclin associated with the CDK-activating kinase MO15. Nature 371, 254–257 (1994). https://doi.org/10.1038/371254a0
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DOI: https://doi.org/10.1038/371254a0
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