Abstract
Mutations in the X-linked MECP2 gene, which encodes the transcriptional regulator methyl-CpG-binding protein 2 (MeCP2), cause Rett syndrome and several neurodevelopmental disorders including cognitive disorders, autism, juvenile-onset schizophrenia and encephalopathy with early lethality. Rett syndrome is characterized by apparently normal early development followed by regression, motor abnormalities, seizures and features of autism, especially stereotyped behaviours. The mechanisms mediating these features are poorly understood. Here we show that mice lacking Mecp2 from GABA (γ-aminobutyric acid)-releasing neurons recapitulate numerous Rett syndrome and autistic features, including repetitive behaviours. Loss of MeCP2 from a subset of forebrain GABAergic neurons also recapitulates many features of Rett syndrome. MeCP2-deficient GABAergic neurons show reduced inhibitory quantal size, consistent with a presynaptic reduction in glutamic acid decarboxylase 1 (Gad1) and glutamic acid decarboxylase 2 (Gad2) levels, and GABA immunoreactivity. These data demonstrate that MeCP2 is critical for normal function of GABA-releasing neurons and that subtle dysfunction of GABAergic neurons contributes to numerous neuropsychiatric phenotypes.
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Acknowledgements
We thank G. Schuster for pronuclear injections; C. Spencer and R. Paylor for advice on behavioural assays; M. Albright for advice on slice electrophysiology; R. Atkinson, Y. Sun, J. Tang and S. Vaishnav for technical advice; V. Brandt for editorial advice. This work was supported by the Howard Hughes Medical Institute, the National Institute of Neurological Disorders and Stroke (NINDS) HD053862, the Simons Foundation, the Rett Syndrome Research Trust (H.Y.Z.); the Intellectual and Developmental Disability Research Centers HD024064 (H.Y.Z., C.R. and J. L. Noebels); NINDS 29709 (J. L. Noebels); the International Rett Syndrome Foundation (C.R.); Autism Speaks (R.C.S.); the National Institute of Mental Health F31MH078678, Baylor Research Advocates for Student Scientists and McNair Fellowships (H.-T.C.).
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H.-T.C. and H.Y.Z. conceived the study. H.-T.C., M.X., C.R. and H.Y.Z. designed experiments with input from H.C., R.C.S., J. L. Neul, H.-C.L. and J. L. Noebels. H.-T.C., H.C., R.C.S., M.X., M.C., J.Y. and J. L. Neul performed experiments. H.-T.C., H.C., M.X., J.Y. and J. L. Neul analysed data; H.-T.C., M.X., C.R. and H.Y.Z. interpreted data with input from H.C., R.C.S., J.Y., J. L. Neul, H.-C.L. and J. L. Noebels. S.G. and N.H. provided reagents for generation of Viaat–Cre; J.L.R.R. and M.E. provided Dlx5/6–Cre mice. H.-T.C., M.X. and H.Y.Z. wrote the manuscript and H.C., R.C.S., M.C., J.L. Neul, S.G., J.L.R.R, J. L. Noebels and C.R. provided input.
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Chao, HT., Chen, H., Samaco, R. et al. Dysfunction in GABA signalling mediates autism-like stereotypies and Rett syndrome phenotypes. Nature 468, 263–269 (2010). https://doi.org/10.1038/nature09582
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DOI: https://doi.org/10.1038/nature09582
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