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Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo

Nature Biotechnology volume 15, pages 871–875 (1997)Cite this article

Abstract

Retroviral vectors derived from lentiviruses such as HIV-1 are promising tools for human gene therapy because they mediate the in vivo delivery and long-term expression of transgenes in nondividing tissues. We describe an HIV vector system in which the virulence genes env, vif, vpr, vpu, and nef have been deleted. This multiply attenuated vector conserved the ability to transduce growth-arrested cells and monocyte-derived macrophages in culture, and could efficiently deliver genes in vivo into adult neurons. These data demonstrate the potential of lentiviral vectors in human gene therapy.

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Author information

Author notes

  1. Romain Zufferey & Didier Trono

    Present address: Department of Genetics and Microbiology, CMU, 9 Avenue de Champel, CH-1211, Geneve, 4, Switzerland

Authors and Affiliations

  1. The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA, 92037-1099

    Romain Zufferey & Didier Trono

  2. Cell Genesys, Foster City, CA, 94404

    Dea Nagy, Ron J. Mandel & Luigi Naldini

Authors
  1. Romain Zufferey
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  2. Dea Nagy
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  3. Ron J. Mandel
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  4. Luigi Naldini
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  5. Didier Trono
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Correspondence to Didier Trono.

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Zufferey, R., Nagy, D., Mandel, R. et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 15, 871–875 (1997). https://doi.org/10.1038/nbt0997-871

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