Abstract
Regulation of AMPA receptor (AMPAR) trafficking is important for neural plasticity. Here we examined the trafficking and synthesis of the GluR1 and GluR2 subunits using ReAsH-EDT2 and FlAsH-EDT2 staining. Activity blockade of rat cultured neurons increased dendritic GluR1, but not GluR2, levels. Examination of transected dendrites revealed that both AMPAR subunits were synthesized in dendrites and that activity blockade enhanced dendritic synthesis of GluR1 but not GluR2. In contrast, acute pharmacological manipulations increased dendritic synthesis of both subunits. AMPARs synthesized in dendrites were inserted into synaptic plasma membranes and, after activity blockade, the electrophysiological properties of native synaptic AMPARs changed in the manner predicted by the imaging experiments. In addition to providing a novel mechanism for synaptic modifications, these results point out the advantages of using FlAsH-EDT2 and ReAsH-EDT2 for studying the trafficking of newly synthesized proteins in local cellular compartments such as dendrites.
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Acknowledgements
We thank J. Fisher and E. Saura for technical assistance and members of the Malenka and Garner labs for suggestions. cDNAs were gifts from M. Sheng (AMPAR subunits), Z. Jia (AMPAR subunits), H. Schulman (α and βCaMKII) and M. Mayford (α and βCaMKII). This work was supported by grants from the National Institutes of Health (R.C.M., MH63394; R.Y.T., NS27177; M.E., P41-RR04050) and Howard Hughes Medical Institute (R.Y.T.).
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R.Y. Tsien is co-inventor on the patents for FlAsH and ReAsH, which the University of California has licensed, and will receive a share of any royalties that the University of California collects. He has no financial relationship with Invitrogen, the company that is now commercializing these reagents.
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Ju, W., Morishita, W., Tsui, J. et al. Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors. Nat Neurosci 7, 244–253 (2004). https://doi.org/10.1038/nn1189
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DOI: https://doi.org/10.1038/nn1189
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