ABSTRACT
A wide variety of neurons and glial cells differentiate from common precursor cells in the developing nervous system. During this process, Notch-mediated cell-cell interaction is essential for maintenance of dividing cells and subsequent generation of cell type diversity. Activation of Notch inhibits cellular differentiation, and abnormality of the Notch pathway leads to premature neuronal differentiation, the lack of some cell types, and severe defects of tissue morphogenesis. Recent data demonstrate that Notch fails to inhibit cellular differentiation in the absence of the bHLH genes Hes1 and Hes5, which functionally antagonize the neuronal bHLH genes such as Mash1. These results indicate that the two Hes genes are essential effectors for the Notch pathway and that neuronal differentiation is controlled by the pathway “NotchHes1/Hes5-|Mash1”.
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INTRODUCTION
During mammalian neural development, a wide variety of neurons and glial cells differentiate from common precursor cells. For example, in the retina, a part of the central nervous system, six types of neurons and one type of glial cells differentiate from common retinal precursor cells1. This differentiation proceeds according to the cell type-specific kinetics: some cell types such as ganglion cells differentiate only at early stages but others such as rod photoreceptors at later stages, probably responding to the different inductive cues in the environment. Generation of such cell type diversity as well as normal morphogenesis involves the cell-cell interaction process, so called “lateral inhibition”. During this process, early-differentiating cells send a signal to the neighboring cells to inhibit them from differentiating into the same cell types2. The transmembrane protein Notch plays an essential role in the lateral inhibition process: Differentiating cells express the Notch ligands (Delta, Jagged, Serrate) on the cell surface and activate Notch of the neighboring cells. Notch activation inhibits cellular differentiation, thereby maintaining dividing precursor cells and enabling the inhibited cells to adopt different cell types at later stages3. In the absence of the Notch pathway, dividing cells decrease and the majority of, or all, cells prematurely differentiate into the early-born cell types, resulting in small-sized disorganized tissues and the lack of late-born cell types. Thus, the Notch-dependent lateral inhibition is critical to generate correct tissue morphogenesis and cell type diversity, although the mechanism for inhibition of differentiation by Notch still remains to be analyzed. Recent data demonstrated that basic helix-loop-helix (bHLH) genes, Hes1 and Hes5 (mammalian homologues of Drosophila hairy and Enhancer of split), play an important role in the Notch pathway3, 4, 5. In this review, we describe the current view of the mammalian Notch pathway.
The Notch pathway: cleavage and translocation of Notch
Notch is a transmembrane protein with epidermal growth factor (EGF) repeats in the extracellular domain and ankyrin repeats in the intracellular domain (Fig 1). There are, at least, four different but related Notch genes (Notch1 to 4) in mice, which show distinct spatio-temporal expression patterns. Notch is processed at the extracellular domain by a furin-like protease and present as a heterodimeric molecule (Fig 1)2. This Notch molecule can be activated by its ligands expressed by neighboring cells. There are several Notch ligands in mice: Delta, Jagged, and Serrate. These ligands also have EGF repeats at the extracellular domain like Notch. When Notch is activated by its ligands, the intracellular domain (ICD) of Notch is likely to be cleaved by γ-secretase (Fig 1)6,7. This protease may be encoded by presenilin1, a gene responsible for Alzheimer's disease (AD)7. In AD patients, the affected Presenilin1 seems to cleave β-amyloid precursor protein to generate amyloid-b peptide, which is deposited in the brains of AD patients.
After cleavage of Notch by γ-secretase, Notch ICD is then translocated into the nucleus and forms a complex with the DNA-binding protein RBP-J9. When RBP-J-binding sites in the Hes1 promoter were disrupted, the complex cannot interact with the promoter and Hes1 upregulation was completely abolished (Fig 2, Hes1/RBP-J(-)). Since Hes1 and Hes5 are known to inhibit neuronal differentiation10, the above data suggest that the two Hes genes may mediate Notch-induced inhibition of cellular differentiation.
Interestingly, although four Notch genes have a conserved structure, recent analysis indicated that Notch1 and Notch3 are functionally different. While Notch1 ICD upregulates Hes1 promoter activity very efficiently as mentioned above, Notch3 ICD does not. Or rather, Notch3 ICD represses Notch1 ICD-dependent transcriptional activation11. Hes5 expression is thus repressed in mouse embryos overexpressing Notch3 ICD. Notch3 ICD seems to compete with Notch1 ICD for a common coactivator as well as RBP-J11. Thus, in some cases Hes expression is subject to both positive and negative regulation by the Notch activation.
Hes1 and Hes5: transcriptional repressors
Hes1 and Hes5 encode bHLH factors that repress transcription by two different mechanisms12, 13. One mechanism is “active repression” mediated by corepressor Groucho (Fig 3A). Both Hes1 and Hes5, which can bind to the N box sequence (CACNAG), have the four-amino-acid sequence WRPW at the carboxyl terminus. The corepressor Groucho, which actively represses transcription, interacts with this WRPW sequence14. Thus, in association with Groucho, Hes1 and Hes5 directly bind to the N box and actively repress gene expression. For example, Hes1 is shown to bind to the N box-related sequence of the Mash1 promoter and repress Mash1 transcription15. The other mechanism is a dominant-negative regulation (Fig 3B). Most bHLH factors such as Mash1 bind to the E box (CANNTG) and activate gene expression. Hes1 and Hes5 form a non-functional heterodimer with such bHLH activators and inhibit their activity12,13. For example, Hes1 dominant-negatively inhibits Mash1-dependent transcription. Thus, Hes1 and Hes5 repress transcription through the N and E boxes by different mechanisms.
Hes1 and Hes5 as Notch effectors
The notion that Notch and Hes function in the same pathway is supported by the analysis of knock-out mice. In Hes1-deficient mice, neurons differentiate prematurely before precursor cells proliferate, resulting in severe defects of the neural tube formation such as anencephaly and abnormalities of eye morphogenesis16,17. In these mice, Mash1 expression prematurely occurs and is also upregulated, suggesting that this Mash1 upregulation may account for premature neuronal differentiation in the absence of Hes1. Similarly, in Hes5-deficient mice neurons prematurely differentiate although Hes5 deficiency seems to be later compensated18. Similar phenotype of premature neuronal differentiation is also observed in both Notch1-deficient and RBP-J-deficient mice19, indicating that Notch, RBP-J and Hes function in the same pathway to prevent premature differentiation.
The definitive evidence for Hes1 and Hes5 as essential Notch effectors is presented by the experiments to determine the Notch effects in the absence of Hes genes18. When the extracellular domain is deleted, Notch is known to function constitutively. When this deletion fragment of Notch is expressed with retrovirus (caNotch-AP, Fig 4A) in neural precursor cells, the virus-infected cells were negative for the neuronal marker MAP2 and formed large clusters without any neurite extension (Fig 4B-D).
Thus, their neuronal differentiation was blocked. caNotch-AP infection still blocked neuronal differentiation of precursor cells prepared from Hes1 knock-out (Fig 4E-G) or Hes5 knock-out mice (Fig 4H-J) but not from Hes1-Hes5 double knock-out mice (Fig 4K-M)18. These results strongly support the conclusion that Hes1 and Hes5 are essential Notch effectors. Thus, neuronal differentiation is controlled by the pathway “Delta-Notch-RBP-J-Hes1/ Hes5-|Mash1”. This negative regulation by Notch and Hes seems to be essential for Mash1 to function at the proper timing. However, some Hes1-Hes5 double-null cells are still inhibited by caNotch-AP infection from differentiating as mature neurons18, suggesting that Notch can still partially function independently of Hes1 and Hes5. The molecular nature of Hes1-Hes5-independent Notch pathway is a current hot topic20,21 and remains to be analyzed.
Inner ear development and the Notch pathway
The importance of the Notch pathway in generation of cell type diversity in mammals is shown by analysis of inner ear development. Hair cells (neurons) and support cells differentiate from common precursors in the inner ear. Ablation of hair cells activates support cells, which first divide and then differentiate into a hair cell and a support cell again, suggesting that support cells receive an inhibitory signal from neighboring hair cells. Actually, hair cells express Jagged2, one of Notch ligands, and support cells express Notch, indicating that hair cells inhibit the neighboring support cells from differentiating as hair cells through the Notch pathway (Fig 5)22. In Jagged2 deficient mice, this inhibition is affected, resulting in the increase of hair cells at the expense of support cells22. This Jagged2-dependent Notch activation probably targets to the bHLH gene Math1, which is essential for generation of hair cells23, 24. Thus, in the inner ear the pathway “Jagged2-Notch- RBPJ-Hes1/Hes5-|Math1” regulates generation of hair cells and support cells, and abnormality of this pathway leads to the lack of one of the two cell types.
The Notch-Hes pathway in other cell types
The Notch-Hes pathway is involved in differentiation of other tissues and cell types as well, such as blood cells, endocrine-exocrine cells, and somites. For example, this pathway plays an essential role in T cell development; In the absence of either Notch1 or Hes1, T cell development is arrested at the earliest phase before T cell receptor gene rearrangement, indicating that both Notch1 and Hes1 are essential for T cell fate specification25, 26. In addition, both Notch1 and Hes1 also regulate later stages of T cell development such as generation of CD8 single-positive cells from CD4CD8 double-positive cells27,28. Interestingly, gene disruption of Notch1 or Hes1 does not affect B cell development.
In addition, the abnormality of the Notch pathway is shown to be involved in various diseases including leukemia and hereditary neurological disorders such as CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy)29,30. However, it remains to be determined whether Hes genes are also involved in these diseases. Further characterization of the Notch pathway should provide insight into the mechanism and therapy for such diseases.
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Acknowledgements
This work was supported by Special Coordination Funds for Promoting Science and Technology and research grants from the Ministry of Education, Science, Sports, and Culture of Japan and Japan Society for the Promotion of Science.
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KAGEYAMA, R., OHTSUKA, T. The Notch-Hes pathway in mammalian neural development. Cell Res 9, 179–188 (1999). https://doi.org/10.1038/sj.cr.7290016
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DOI: https://doi.org/10.1038/sj.cr.7290016
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