Abstract
Previous data have shown that in several tumor cells lines the addition of heregulin results in the translocation of ErbB-4 to a detergent-insoluble membrane fraction where it is hypertyrosine phosphorylated. The data herein demonstrate that heregulin or betacellulin, but not EGF, promotes the rapid translocation of ErbB-2, the heterodimerization partner for ErbB-4, to the same detergent-insoluble fraction in T47 D mammary carcinoma cells. The translocation of ErbB-2 and ErbB-4, but not ErbB-3, to this detergent-insoluble fraction is readily detected 2 min after the addition of heregulin. ErbB-2 present in this detergent-insoluble membrane fraction is tyrosine phosphorylated to a level fourfold greater than the majority of cellular ErbB-2 present in the detergent-soluble membrane fraction. These results suggest the ligand-dependent formation of hyperphosphorylated ErbB-4/ErbB-2 dimers coordinate with translocation to a putative membrane microdomain.
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References
Alroy I, Yarden Y . 1997 FEBS Lett. 410: 83–86
Burden S, Yarden Y . 1997 Neuron 18: 847–855
Carpenter G . 2000 BioEssays 22: 697–707
Dunbar AJ, Goddard C . 2000 Int. J Biochem. Cell Biol. 32: 805–815
Holowka D, Sheets ED, Baird B . 2000 J. Cell Sci. 113: 1009–1019
Hooper NM . 1999 Mol. Mem. Biol. 16: 145–165
Leupin O, Zaru R, Laroche T, MĂ¼ller S, Valitutti S . 2000 Curr. Biol. 10: 277–280
Mineo C, Gill GN, Anderson RGW . 1999 J. Biol. Chem. 274: 30636–30643
Montixi C, Langlet C, Bernard A-M, Thimonier J, Dubois C, Wurbel M-A, Chauvin J-P, Pierres M, He H-T . 1998 EMBO J. 17: 5334–5348
Moran M, Miceli MC . 1998 Immunity 9: 787–796
Okamoto T, Schlegel A, Scherer PE, Lisanti MP . 1998 J. Biol. Chem. 273: 5419–5422
Oliferenko S, Paiha K, Harder T, Gerke V, Schwärzler C, Schwarz H, Beug H, GĂ¼nthert U, Huber LA . 1999 J. Cell Biol. 146: 843–854
Riese II DJ, Stern DF . 1998 Bio Essays 20: 41–48
Simons K, Toomre D . 2000 Nature Rev. Mol. Cell Biol. 1: 31–39
Yarden Y, Sliwkowski MX . 2001 Nature Rev. Mol. Cell Biol. 2: 127–137
Zhao Y-Y, Feron O, Dessy C, Han X, Marchionni MA, Kelly RA . 1999 Cir. Res. 84: 1380–1387
Zhou W, Carpenter G . 2000 J. Biol. Chem. 275: 34737–34743
Acknowledgements
The authors appreciate the efforts of Sue Carpenter in manuscript preparation. This work was supported by NIH grant RO1 CA24071 and T32 CA09582.
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Zhou, W., Carpenter, G. Heregulin-dependent translocation and hyperphosphorylation of ErbB-2. Oncogene 20, 3918–3920 (2001). https://doi.org/10.1038/sj.onc.1204517
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DOI: https://doi.org/10.1038/sj.onc.1204517
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