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Exchange of catenins in cadherin–catenin complex

Oncogene volume 22, pages 1181–1188 (2003)Cite this article

Abstract

β-Catenin is an intracellular multifunctional protein. In complex with the transmembrane adhesive receptor E-cadherin, it becomes plasma membrane-associated and mediates intercellular adhesion. A cytosolic pool of β-catenin interacts with DNA-binding proteins and participates in signal transduction. To reveal the possible cross-talk between these two pools, we studied whether β-catenin is exchanged between its free and cadherin-bound states. We found that pulse-labeled β-catenin replaces the β-catenin bound to the cell surface prebiotinylated E-cadherin immediately after synthesis. Approximately 25% of all pulse-labeled β-catenin destined for E-cadherin associates with this protein via this mechanism. The rest of the newly synthesized β-catenin arrives at the plasma membrane in a complex with the E-cadherin precursor. Immediately after arrival, this β-catenin pool is transferred to the prebiotinylated E-cadherin. β-Catenin released from E-cadherin may participate in new exchange cycles. This β-catenin exchange is strongly affected in cells that contain mutations in the tumor suppressor gene APC. This process may contribute significantly to both cell–cell adhesion and β-catenin-dependent signaling.

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Acknowledgements

We thank Drs A Eizen (Washington University, St Louis, MO, USA), A Ljubimov (Cedars-Sinai Medical Center, Los Angeles, CA, USA), and N Gloushankova (Russian Cancer Research Center, Moscow, Russia) for valuable discussions. This work has been supported by National Institutes of Health Grants AR44016-04 and AR45254-01.

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  1. Jörg Klingelhöfer

    Present address: Department of Molecular Oncology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK - 2100, Copenhagen, Denmark

  2. Oscar Y Laur

    Present address: Department of Pathology and Laboratory Medicine, Emory University Medical School, WMB 7302, 1639 Pierce Dr., Atlanta, GA, 30322, USA

Authors and Affiliations

  1. Division of Dermatology, Washington University Medical School, St Louis, 63110, MO, USA

    Jörg Klingelhöfer, Regina B Troyanovsky, Oscar Y Laur & Sergey Troyanovsky

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  1. Jörg Klingelhöfer
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Correspondence to Sergey Troyanovsky.

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Klingelhöfer, J., Troyanovsky, R., Laur, O. et al. Exchange of catenins in cadherin–catenin complex. Oncogene 22, 1181–1188 (2003). https://doi.org/10.1038/sj.onc.1206245

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