Basic—Alimentary TractInhibition of p38 Mitogen-Activated Protein Kinase Pathway as Prophylaxis of Postoperative Ileus in Mice
Section snippets
Animals
Male C57BL/6J mice (∼25g body weight) were obtained from Harlan–Winkelmann (Borchen, Germany). CSF-1 mutant op−/− mice (strain B6C3Fe a/a-Csf1op) and heterozygous (op+/−) of mixed gender were used at an age of 6 weeks and bred as described before.7 The op−/− mice were phenotyped by the absence of incisors at day P10. All experiments were performed in accordance with the federal law regarding the protection of animals. The principles of laboratory animal care were followed. Animals were
p38-MAPK Activation
MAPKs are known to play a pivotal role in transmission of proinflammatory and mechanical stress stimuli in various diseases. In POI, a mechanical trauma initiates a massive inflammatory reaction within the ME of the intestinal wall. We tested the activation of different MAPK pathways after abdominal surgery and IM. Activation of p38-MAPK by phosphorylation was detected 15 minutes after IM in ME lysates, attenuating 30 minutes postoperatively and declining to near-control levels after 1 hour (
Discussion
POI is a problem of every abdominal operative procedure.2 The surgical trauma initiates a complex and severe inflammation within the gut tunica muscularis (ME), resulting in smooth muscle dysfunction and, subsequently, gastrointestinal dysmotility.3, 4, 6, 13, 14 Recently, we showed that a network of resident macrophages within the ME is one of the central players in the initiation of this proinflammatory reaction.7 This study extends our previous work targeting the inhibition of
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2018, Cellular Immunology6-Gingerol protects intestinal barrier from ischemia/reperfusion-induced damage via inhibition of p38 MAPK to NF-κB signalling
2017, Pharmacological ResearchCitation Excerpt :p38 mitogen activated protein kinase (p38 MAPK) signaling pathway mediates inflammatory response, apoptosis, and differentiation under stress conditions, including I/R injury [14–16]. And under stress conditions, the intracellular p38 can be transferred to the nucleus, and the expression of genes involved in regulation of transcription factors is regulated by phosphorylation [17,18]. A growing number of reports suggest that interference with p38 MAPK function may decrease the extent of tissue injury in disease models, including intestinal injury model [19–21].
The efficacy and results of medical treatment in postoperative ileus
2023, Nigerian Journal of Clinical PracticeInflammation and Impaired Gut Physiology in Post-operative Ileus: Mechanisms and the Treatment Options
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The authors disclose the following: Supported by grants from the DFG (KA1270/3-1/2) and BONFOR (O-112.0034). Vidal F. de la Cruz and Thais Sielecki are employees of Cytokine PharmaSciences, Inc, and are inventors on several patents and applications relating to semapimod that are owned by Cytokine PharmaSciences, Inc.