Autoradiographic evidence that intrastriatal administration of adenosine A(1) receptor antisense oligodeoxynucleotide decreases adenosine A(1) receptors in the rat striatum and cortex

Brain Res Mol Brain Res. 1999 Oct 1;72(2):226-30. doi: 10.1016/s0169-328x(99)00225-9.

Abstract

In this study, the effect of a phosphorothioated A(1) adenosine receptor antisense oligodeoxynucleotide on A(1) receptor density and mRNA in the striatum and cortex of rats was determined. Receptor autoradiography and in situ hybridization revealed a reduction in striatal and cortical A(1) receptor density and cortical A(1) receptor mRNA, respectively, in antisense-treated brains but not in those treated with a mismatch oligonucleotide. There was no change in A(2) receptor binding. These data imply that the corticostriatal pathway synthesizes A(1) receptors and transports them to its terminals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine / pharmacology
  • Animals
  • Autoradiography
  • Axonal Transport
  • Cerebral Cortex / metabolism*
  • Corpus Striatum / metabolism*
  • Depression, Chemical
  • Diffusion
  • Gene Expression Regulation / drug effects*
  • In Situ Hybridization
  • Injections
  • Male
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Oligodeoxyribonucleotides, Antisense / pharmacology*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Purinergic P1 / biosynthesis
  • Receptors, Purinergic P1 / genetics*
  • Thionucleotides / pharmacology*

Substances

  • Nerve Tissue Proteins
  • Oligodeoxyribonucleotides, Antisense
  • RNA, Messenger
  • Receptors, Purinergic P1
  • Thionucleotides
  • Adenosine