Abstract
In this study, the effect of a phosphorothioated A(1) adenosine receptor antisense oligodeoxynucleotide on A(1) receptor density and mRNA in the striatum and cortex of rats was determined. Receptor autoradiography and in situ hybridization revealed a reduction in striatal and cortical A(1) receptor density and cortical A(1) receptor mRNA, respectively, in antisense-treated brains but not in those treated with a mismatch oligonucleotide. There was no change in A(2) receptor binding. These data imply that the corticostriatal pathway synthesizes A(1) receptors and transports them to its terminals.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenosine / pharmacology
-
Animals
-
Autoradiography
-
Axonal Transport
-
Cerebral Cortex / metabolism*
-
Corpus Striatum / metabolism*
-
Depression, Chemical
-
Diffusion
-
Gene Expression Regulation / drug effects*
-
In Situ Hybridization
-
Injections
-
Male
-
Nerve Tissue Proteins / biosynthesis
-
Nerve Tissue Proteins / genetics*
-
Oligodeoxyribonucleotides, Antisense / pharmacology*
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Rats
-
Rats, Sprague-Dawley
-
Receptors, Purinergic P1 / biosynthesis
-
Receptors, Purinergic P1 / genetics*
-
Thionucleotides / pharmacology*
Substances
-
Nerve Tissue Proteins
-
Oligodeoxyribonucleotides, Antisense
-
RNA, Messenger
-
Receptors, Purinergic P1
-
Thionucleotides
-
Adenosine