Abstract
The involvement of protein kinases A and C in the induction of low frequency stimulation-induced long-term depression (LTD) in the medial perforant path of the dentate gyrus in vitro has been studied using the selective PKA inhibitors H-89 and KT 5720 and PKC inhibitors Bisindolylmaleimide and Ro-31-8220. The PKC inhibitors Bisindolylmaleimide I and Ro-31-8220 and the PKA inhibitors H-89 and KT5720 all partially inhibited LTD induction. However, the presence of both a PKC and a PKA inhibitor was necessary to completely block LTD induction. The induction of long-term potentiation was not blocked by the inhibitors. It is suggested that the induction of LTD by LFS involves activation of PKC and PKA following activation of group I and group II metabotropic glutamate receptors (mGluR).
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Carbazoles*
-
Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
-
Cyclic AMP-Dependent Protein Kinases / metabolism*
-
Dentate Gyrus / chemistry
-
Dentate Gyrus / enzymology
-
Enzyme Inhibitors / pharmacology
-
Excitatory Postsynaptic Potentials / drug effects
-
Excitatory Postsynaptic Potentials / physiology
-
In Vitro Techniques
-
Indoles / pharmacology
-
Isoquinolines / pharmacology
-
Long-Term Potentiation / physiology*
-
Maleimides / pharmacology
-
Neural Inhibition / physiology
-
Perforant Pathway / chemistry
-
Perforant Pathway / enzymology*
-
Protein Kinase C / antagonists & inhibitors
-
Protein Kinase C / metabolism*
-
Pyrroles / pharmacology
-
Rats
-
Receptors, Metabotropic Glutamate / physiology*
-
Sulfonamides*
Substances
-
Carbazoles
-
Enzyme Inhibitors
-
Indoles
-
Isoquinolines
-
Maleimides
-
Pyrroles
-
Receptors, Metabotropic Glutamate
-
Sulfonamides
-
KT 5720
-
Cyclic AMP-Dependent Protein Kinases
-
Protein Kinase C
-
N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
-
bisindolylmaleimide
-
Ro 31-8220