Abstract
Reactive oxygen species (ROS) are necessary for programmed cell death (PCD) in neurons, but the underlying ROS-producing enzymes have not been identified. NADPH oxidase produces ROS, although the expression of its five subunits are thought to be restricted largely to non-neuronal cells. Here, we show that NADPH oxidase subunits are present in neurons. Moreover, both an NADPH oxidase inhibitor, diphenyleneiodonium, and NAPDH oxidase genetic deficiency inhibit apoptosis in a classic model of PCD, i.e., NGF-deprived sympathetic neurons. Overall, these results indicate that NADPH oxidase is unexpectedly present in neurons and can contribute to neuronal apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Apoptosis / physiology*
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Cells, Cultured
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Enzyme Inhibitors / pharmacology
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Immunohistochemistry
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Mice
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NADPH Oxidases / antagonists & inhibitors
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NADPH Oxidases / metabolism*
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Nerve Growth Factor / deficiency*
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Nerve Growth Factor / genetics
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Neurons / cytology
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Neurons / enzymology
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Neurons / metabolism*
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Onium Compounds / pharmacology
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Oxidative Stress*
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RNA, Messenger / metabolism
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Reactive Oxygen Species / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sympathetic Nervous System / cytology
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Sympathetic Nervous System / enzymology
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Sympathetic Nervous System / metabolism*
Substances
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Enzyme Inhibitors
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Onium Compounds
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RNA, Messenger
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Reactive Oxygen Species
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diphenyleneiodonium
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Nerve Growth Factor
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NADPH Oxidases