Abstract
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified*
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Fertilization
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Gene Transfer Techniques
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Male
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Ovum
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Sperm Injections, Intracytoplasmic
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Spermatozoa
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Xenopus / genetics*
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Xenopus laevis
Substances
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Luminescent Proteins
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Green Fluorescent Proteins