Abstract
Sensory transduction for many taste stimuli such as sugars, some bitter compounds and amino acids is thought to be mediated via G protein-coupled receptors (GPCRs), although no such receptors that respond to taste stimuli are yet identified. Monosodium L-glutamate (L-MSG), a natural component of many foods, is an important gustatory stimulus believed to signal dietary protein. We describe a GPCR cloned from rat taste buds and functionally expressed in CHO cells. The receptor couples negatively to a cAMP cascade and shows an unusual concentration-response relationship. The similarity of its properties to MSG taste suggests that this receptor is a taste receptor for glutamate.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism
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CHO Cells
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Chemoreceptor Cells / metabolism*
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Cloning, Molecular
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Colforsin / pharmacology
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Cricetinae
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Cyclic AMP / metabolism
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DNA, Complementary / genetics
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Dose-Response Relationship, Drug
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GTP-Binding Proteins / metabolism
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Glutamic Acid / metabolism*
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Glutamic Acid / pharmacology
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Molecular Sequence Data
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Organ Specificity
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Polymerase Chain Reaction
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Propionates / pharmacology
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Protein Isoforms / genetics
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Protein Isoforms / metabolism*
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Metabotropic Glutamate / genetics
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Receptors, Metabotropic Glutamate / metabolism*
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Taste / physiology*
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Taste Buds / metabolism*
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Transfection
Substances
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2-amino-4-phosphono-propinate
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DNA, Complementary
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Propionates
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Protein Isoforms
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RNA, Messenger
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Receptors, Metabotropic Glutamate
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Colforsin
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Glutamic Acid
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Cyclic AMP
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GTP-Binding Proteins
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metabotropic glutamate receptor 4