Activation of a capacitative Ca(2+) entry pathway by store depletion in cultured hippocampal neurones

FEBS Lett. 2000 Mar 31;470(3):269-72. doi: 10.1016/s0014-5793(00)01340-5.

Abstract

Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both transiently elevated [Ca(2+)](i). A subsequent addition of Ca(2+) into the bathing medium caused a second [Ca(2+)](i) change which was blocked by lanthanum (50 microM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca(2+) entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca(2+) entry in excitable cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
  • Aniline Compounds / metabolism
  • Animals
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Calcium Signaling / drug effects
  • Calcium-Transporting ATPases / antagonists & inhibitors
  • Calcium-Transporting ATPases / metabolism
  • Carbachol / pharmacology
  • Cells, Cultured
  • Cholinergic Agonists / pharmacology
  • Electric Conductivity
  • Excitatory Amino Acid Antagonists / pharmacology
  • Hippocampus / cytology*
  • Hippocampus / metabolism
  • Lanthanum / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Potassium / pharmacology
  • Rats
  • Tetrodotoxin / pharmacology
  • Thapsigargin / pharmacology
  • Xanthenes / metabolism

Substances

  • Aniline Compounds
  • Cholinergic Agonists
  • Excitatory Amino Acid Antagonists
  • Xanthenes
  • Fluo-3
  • Tetrodotoxin
  • Thapsigargin
  • Lanthanum
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • Carbachol
  • Calcium-Transporting ATPases
  • Potassium
  • Calcium