Abstract
Dendritic localization of the alpha subunit of Ca2+/calmodulin-dependent protein kinase II (alphaCaMKII) mRNA in CNS neurons requires its 3' untranslated region (3'UTR). We investigated this targeting mechanism by identifying two cis-acting elements in the 3'UTR. One is a 30-nucleotide element that mediated dendritic translocation. A homologous sequence in the 3'UTR of neurogranin, transcripts of which also reside in dendrites, also funtioned in cis to promote its dendritic transport. Other putative elements in the alphaCaMKII mRNA inhibit its transport in a resting state. This inhibition was removed in depolarized neurons, and such activity-dependent derepression was a primary requirement for their dendritic targeting.
MeSH terms
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Animals
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Base Sequence / genetics
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Calcium-Calmodulin-Dependent Protein Kinase Type 2
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Calcium-Calmodulin-Dependent Protein Kinases / genetics*
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Calmodulin-Binding Proteins / genetics*
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Calmodulin-Binding Proteins / metabolism
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Cells, Cultured
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Cloning, Molecular / methods
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Dendrites / genetics*
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Dendrites / metabolism
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Embryo, Mammalian
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Gene Expression Profiling / methods
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Hippocampus
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Memory / physiology
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Neurogranin
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RNA, Messenger / genetics*
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Rats
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Rats, Wistar
Substances
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Calmodulin-Binding Proteins
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Nerve Tissue Proteins
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Nrgn protein, rat
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RNA, Messenger
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Neurogranin
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Calcium-Calmodulin-Dependent Protein Kinase Type 2
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Calcium-Calmodulin-Dependent Protein Kinases