Postsynaptic targeting of MAGUKs mediated by distinct N-terminal domains

Neuroreport. 2000 Nov 9;11(16):3479-84. doi: 10.1097/00001756-200011090-00016.

Abstract

Postsynaptic targeting of PSD-95 has been extensively studied; however, little is known about how other MAGUKs are localized. Proper targeting of PSD-95 requires dual palmitoylation of an N-terminal motif. We now find that the N-termini of closely related PSD-93 and SAP-102 are also involved in postsynaptic targeting. PSD-93 is N-terminally palmitoylated; however, unlike PSD-95, palmitoylation does not explain the necessity of the N-terminus for PSD-93 postsynaptic targeting. Furthermore, when the N-terminus of PSD-95 is replaced with the first 30 or 64, but not the first 10, amino acids of PSD-93, the chimera is targeted to postsynaptic sites independent of palmitoylation. Similarly, when the N-terminus of PSD-95 is replaced with the non-palmitoylated N-terminus of SAP-102, postsynaptic targeting is maintained. These results suggest that MAGUKs contain diverse signals within their N-termini for postsynaptic targeting.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Disks Large Homolog 4 Protein
  • Embryo, Mammalian
  • Guanylate Kinases
  • Hippocampus / cytology
  • Hippocampus / physiology
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • Neurons / cytology
  • Neurons / physiology*
  • Neuropeptides / chemistry
  • Neuropeptides / metabolism*
  • Nucleoside-Phosphate Kinase / metabolism*
  • Palmitic Acid / metabolism
  • Rats
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Synapses / physiology*
  • Synapses / ultrastructure
  • Transfection
  • Tumor Suppressor Proteins

Substances

  • Disks Large Homolog 4 Protein
  • Dlg3 protein, rat
  • Dlg4 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mpp2 protein, rat
  • Nerve Tissue Proteins
  • Neuropeptides
  • Recombinant Proteins
  • Tumor Suppressor Proteins
  • postsynaptic density proteins
  • Palmitic Acid
  • Nucleoside-Phosphate Kinase
  • DLG2 protein, human
  • Guanylate Kinases