Cloning and characterization of RTK ligands using receptor-alkaline phosphatase fusion proteins

Methods Mol Biol. 2001:124:313-34. doi: 10.1385/1-59259-059-4:313.

Authors

H J Cheng¹, J G Flanagan

Affiliation

¹ Howard Hughes Medical Institute, Department of Anatomy, University of California at San Francisco, USA.

PMID: 11100484
DOI: 10.1385/1-59259-059-4:313

No abstract available

MeSH terms

3T3 Cells
Alkaline Phosphatase / genetics
Alkaline Phosphatase / metabolism
Amino Acid Sequence
Animals
Base Sequence
COS Cells
Cell Line
Cell-Free System
Chlorocebus aethiops
Cloning, Molecular / methods
Embryo, Mammalian
Escherichia coli
Genetic Vectors
Humans
Ligands
Mice
Phosphoproteins / isolation & purification
Phosphoproteins / metabolism*
Receptor Protein-Tyrosine Kinases / genetics
Receptor Protein-Tyrosine Kinases / metabolism*
Recombinant Fusion Proteins / metabolism
Restriction Mapping
Transfection

Substances

Ligands
Phosphoproteins
Recombinant Fusion Proteins
Receptor Protein-Tyrosine Kinases
Alkaline Phosphatase