Abstract
Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca(2+) during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca(2+) and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Calcium / metabolism
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Calcium / physiology*
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Cells, Cultured
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Chemotaxis
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Cyclic AMP / metabolism
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Cyclic AMP / physiology
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Electric Stimulation
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Glycoproteins / pharmacology*
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Growth Cones / drug effects*
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Growth Cones / physiology
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Myelin Sheath / metabolism
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Myelin Sheath / physiology*
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Myelin-Associated Glycoprotein / pharmacology*
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Nerve Growth Factors / pharmacology*
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Nerve Regeneration / drug effects
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Nerve Regeneration / physiology
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Netrin-1
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Neurons / drug effects
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Neurons / physiology
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Semaphorin-3A
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Tumor Suppressor Proteins
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Xenopus
Substances
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Glycoproteins
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Myelin-Associated Glycoprotein
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Nerve Growth Factors
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Semaphorin-3A
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Tumor Suppressor Proteins
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Netrin-1
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Cyclic AMP
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Calcium