Abstract
We used gene targeting to generate mice lacking the M1 muscarinic acetylcholine receptor. These mice exhibit a decreased susceptibility to pilocarpine-induced seizures, loss of regulation of M-current potassium channel activity and of a specific calcium channel pathway in sympathetic neurons, a loss of the positive chronotropic and inotropic responses to the novel muscarinic agonist McN-A-343, and impaired learning in a hippocampal-dependent test of spatial memory.
MeSH terms
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Animals
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Calcium Channels / metabolism*
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Cells, Cultured
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Electrophysiology
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GTP-Binding Proteins / metabolism
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Gene Targeting
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Heart / drug effects
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Heart / physiology*
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Hippocampus / cytology
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Hippocampus / physiology
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Humans
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Learning / physiology
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Memory / physiology
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Mice
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Mice, Knockout
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Muscarinic Agonists / pharmacology
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Neurons / drug effects
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Neurons / physiology*
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Oxotremorine / pharmacology
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Pilocarpine / pharmacology
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Potassium Channels / metabolism*
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Rats
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Receptor, Muscarinic M1
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Receptors, Muscarinic / genetics
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Receptors, Muscarinic / metabolism*
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Seizures / chemically induced
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Signal Transduction / genetics
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Signal Transduction / physiology*
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Telencephalon / cytology
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Telencephalon / physiology
Substances
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Calcium Channels
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Muscarinic Agonists
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Potassium Channels
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Receptor, Muscarinic M1
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Receptors, Muscarinic
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Pilocarpine
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Oxotremorine
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GTP-Binding Proteins