A key problem in developmental neurobiology is how axons home in on their correct target tissue and establish the correct synaptic contacts. Recent work shows that in the developing Drosophila visual system a population of distinct lamina glial cells ensures correct target layer selection of retinal axons. In the absence of lamina neurons, photoreceptor axons terminate their growth in the correct zone, but when glial cell migration into the lamina is disrupted, as in nonstop mutants, growth cones advance into deeper layers of the brain.