Abstract
Use-dependent synapse remodeling is thought to provide a cellular mechanism for encoding durable memories, yet whether activity triggers an actual structural change has remained controversial. We use photoconductive stimulation to demonstrate activity-dependent morphological synaptic plasticity by video imaging of GFP-actin at individual synapses. A single tetanus transiently moves presynaptic actin toward and postsynaptic actin away from the synaptic junction. Repetitive spaced tetani induce glutamate receptor-dependent stable restructuring of synapses. Presynaptic actin redistributes and forms new puncta that label for an active synapse marker FM5-95 within 2 hr. Postsynaptic actin sprouts projections toward the new presynaptic actin puncta, resembling the axon-dendrite interaction during synaptogenesis. Our results indicate that activity-dependent presynaptic structural plasticity facilitates the formation of new active presynaptic terminals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
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Actins / metabolism*
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Aniline Compounds / metabolism
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Animals
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Calcium / metabolism*
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Cells, Cultured
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Cyclic AMP / analogs & derivatives*
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Cyclic AMP / metabolism
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Cyclic AMP / pharmacology
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Electric Stimulation*
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Enzyme Inhibitors / pharmacology
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Excitatory Amino Acid Antagonists / pharmacology
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Fluorescent Dyes / metabolism
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Genes, Reporter
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Green Fluorescent Proteins
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Hippocampus / cytology
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Light
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Video
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Neuronal Plasticity / physiology*
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Neurons / drug effects
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Neurons / metabolism*
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Rats
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Receptors, N-Methyl-D-Aspartate / metabolism
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Recombinant Fusion Proteins / metabolism
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Signal Transduction
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Silicon / chemistry
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Synapses / metabolism*
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Thionucleotides / pharmacology
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Valine / analogs & derivatives*
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Valine / pharmacology
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Xanthenes / metabolism
Substances
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Actins
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Aniline Compounds
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Enzyme Inhibitors
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Excitatory Amino Acid Antagonists
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Fluorescent Dyes
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Luminescent Proteins
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Receptors, N-Methyl-D-Aspartate
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Recombinant Fusion Proteins
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Thionucleotides
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Xanthenes
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Green Fluorescent Proteins
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adenosine-3',5'-cyclic phosphorothioate
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Fluo-3
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6-Cyano-7-nitroquinoxaline-2,3-dione
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2-amino-5-phosphopentanoic acid
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Cyclic AMP
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Valine
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Calcium
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Silicon