Abstract
We examined the expression of hepatocyte growth factor (HGF) mRNA and its receptor, c-met mRNA, in the dorsal root ganglia (DRG) and spinal cord of naive adult rats using in situ hybridization histochemistry (ISHH) and the reverse transcription-polymerase chain reaction (RT-PCR) technique. HGF mRNA was expressed in 25.0% of DRG neurons and 80.5% of HGF mRNA-positive neurons expressed trkA mRNA. In the lumbar spinal cord, c-met mRNA signals were observed in the superficial layer of dorsal horn. These results suggest that the HGF/cMet system may be involved in sensory transmission.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Ganglia, Spinal / cytology
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Gene Expression Profiling
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Hepatocyte Growth Factor / biosynthesis*
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Hepatocyte Growth Factor / genetics
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In Situ Hybridization
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Liver / metabolism
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / genetics
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Neurons, Afferent / metabolism*
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Proto-Oncogene Proteins c-met / biosynthesis
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Proto-Oncogene Proteins c-met / genetics
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Rats
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Rats, Sprague-Dawley
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Receptor, trkA / biosynthesis
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Receptor, trkA / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sensation / physiology
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Spinal Cord / cytology
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Synaptic Transmission / physiology
Substances
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Nerve Tissue Proteins
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RNA, Messenger
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Hepatocyte Growth Factor
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Proto-Oncogene Proteins c-met
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Receptor, trkA