Estrogen induces elevation of cAMP-dependent protein kinase activity in immortalized hippocampal neurons: imaging in living cells

A S Shingo; S Kito

doi:10.1007/s007020200012

Estrogen induces elevation of cAMP-dependent protein kinase activity in immortalized hippocampal neurons: imaging in living cells

J Neural Transm (Vienna). 2002 Feb;109(2):171-4. doi: 10.1007/s007020200012.

Authors

A S Shingo¹, S Kito

Affiliation

¹ Hyogo University School of Health Sciences, Japan. sheala@mcn.ne.jp

PMID: 12075856
DOI: 10.1007/s007020200012

Abstract

17beta-estradiol-stimulated PKA activation in living differentiated H19-7 cells was visualized using DR2, a cell-permeable fluorescent PKA substrate. Estradiol induced marked changes of fluorescence in cytosolic areas and these were inhibited by a PKA inhibitor.

MeSH terms

Animals
Cell Line
Cyclic AMP-Dependent Protein Kinases / biosynthesis*
Enzyme Induction
Estradiol / pharmacology*
Hippocampus / enzymology*
Neurons / enzymology*
Rats

Substances

Estradiol
Cyclic AMP-Dependent Protein Kinases