Caspr3 and caspr4, two novel members of the caspr family are expressed in the nervous system and interact with PDZ domains

Mol Cell Neurosci. 2002 Jun;20(2):283-97. doi: 10.1006/mcne.2002.1110.

Abstract

The NCP family of cell-recognition molecules represents a distinct subgroup of the neurexins that includes Caspr and Caspr2, as well as Drosophila Neurexin-IV and axotactin. Here, we report the identification of Caspr3 and Caspr4, two new NCPs expressed in nervous system. Caspr3 was detected along axons in the corpus callosum, spinal cord, basket cells in the cerebellum and in peripheral nerves, as well as in oligodendrocytes. In contrast, expression of Caspr4 was more restricted to specific neuronal subpopulations in the olfactory bulb, hippocampus, deep cerebellar nuclei, and the substantia nigra. Similar to the neurexins, the cytoplasmic tails of Caspr3 and Caspr4 interacted differentially with PDZ domain-containing proteins of the CASK/Lin2-Veli/Lin7-Mint1/Lin10 complex. The structural organization and distinct cellular distribution of Caspr3 and Caspr4 suggest a potential role of these proteins in cell recognition within the nervous system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Aged
  • Animals
  • Cell Adhesion Molecules, Neuronal*
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Cells, Cultured
  • Chromosomes, Human, Pair 16 / genetics
  • Chromosomes, Human, Pair 9 / genetics
  • DNA, Complementary / analysis
  • Drosophila Proteins*
  • Humans
  • Immunohistochemistry
  • Macromolecular Substances
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification*
  • Mice
  • Middle Aged
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification*
  • Nervous System / cytology
  • Nervous System / metabolism*
  • Neuroglia / cytology
  • Neuroglia / metabolism*
  • Neurons / cytology
  • Neurons / metabolism*
  • Protein Binding / physiology
  • Protein Structure, Tertiary / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / isolation & purification*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • CNTNAP1 protein, human
  • CNTNAP3 protein, human
  • Cell Adhesion Molecules, Neuronal
  • Cntnap1 protein, mouse
  • Cntnap1 protein, rat
  • Cntnap4 protein, mouse
  • DNA, Complementary
  • Drosophila Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Nrx protein, Drosophila
  • RNA, Messenger
  • Receptors, Cell Surface