Abstract
A-kinase anchoring proteins (AKAPs) constitute a family of scaffolding proteins that bind the regulatory subunits of protein kinase A (PKA). AKAP binding to PKA regulates the phosphorylation of various proteins, some of which have been implicated in synaptic plasticity and memory consolidation. Here we show that the regulatory subunits of PKA are colocalized with AKAP150 (an AKAP isoform that is expressed in the brain) in the lateral amygdala (LA) and that infusion to the LA of the peptide St-Ht31, which blocks PKA anchoring onto AKAPs, impairs memory consolidation of auditory fear conditioning.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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A Kinase Anchor Proteins
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Adaptor Proteins, Signal Transducing*
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Amygdala / cytology
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Amygdala / enzymology*
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Animals
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Auditory Pathways / cytology
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Auditory Pathways / enzymology*
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Auditory Perception / physiology
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Binding, Competitive / drug effects
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Binding, Competitive / physiology
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Carrier Proteins / metabolism*
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Cell Compartmentation / drug effects
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Cell Compartmentation / physiology
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Conditioning, Psychological / physiology
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Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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Fear / physiology*
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Macromolecular Substances
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Memory / physiology*
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Minor Histocompatibility Antigens
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Neurons / cytology
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Neurons / enzymology*
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Phosphorylation
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Protein Binding / physiology
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Protein Isoforms / antagonists & inhibitors
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Protein Isoforms / metabolism
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Proto-Oncogene Proteins / pharmacology
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Rats
Substances
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A Kinase Anchor Proteins
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AKAP13 protein, human
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AKAP5 protein, human
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Adaptor Proteins, Signal Transducing
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Akap5 protein, rat
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Carrier Proteins
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Macromolecular Substances
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Minor Histocompatibility Antigens
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Protein Isoforms
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Proto-Oncogene Proteins
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Cyclic AMP-Dependent Protein Kinases